Chronic Administration of Oil Palm (Elaeis guineensis ) Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative Stress and Improves Renal Histopathology and Function in Experimental Diabetes
Oil palm (Elaeis guineensis) leaves extract (OPLE) has antioxidant properties and because oxidative stress contributes to the pathogenesis of diabetic nephropathy (DN), we tested the hypothesis that OPLE prevents diabetes renal oxidative stress, attenuating injury. Sprague-Dawley rats received OPL...
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my.usm.eprints.38447 http://eprints.usm.my/38447/ Chronic Administration of Oil Palm (Elaeis guineensis ) Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative Stress and Improves Renal Histopathology and Function in Experimental Diabetes Rajavel, Varatharajan Abdul Sattar, Munavvar Zubaid Abdulla, Mahmood Ameen M. Kassim, Normadiah Abdullah, Nor Azizan RS1-441 Pharmacy and materia medica Oil palm (Elaeis guineensis) leaves extract (OPLE) has antioxidant properties and because oxidative stress contributes to the pathogenesis of diabetic nephropathy (DN), we tested the hypothesis that OPLE prevents diabetes renal oxidative stress, attenuating injury. Sprague-Dawley rats received OPLE (200 and 500 mg kg−1) for 4 and 12 weeks after diabetes induction (streptozotocin 60mg kg−1). Blood glucose level, body and kidney weights, urine flow rate (UFR), glomerular filtration rate (GFR), and proteinuria were assessed. Oxidative stress variables such as 8-hydroxy-2�-deoxyguanosine (8-OHdG), glutathione (GSH), and lipid peroxides (LPO) were quantified. Renalmorphology was analysed, and plasma transforming growth factor-beta1 (TGF-β1) was measured. Diabetic rats demonstrated increase in blood glucose and decreased body and increased kidney weights. Renal dysfunction (proteinuria, elevations in UFR and GFR) was observed in association with increases in LPO, 8-OHdG, and TGF-β1 and a decrease in GSH. Histological evaluation of diabetic kidney demonstrated glomerulosclerosis and tubulointerstitial fibrosis. OPLE attenuated renal dysfunction, improved oxidative stress markers, and reduced renal pathology in diabetic animals. These results suggest OPLE improves renal dysfunction and pathology in diabetes by reducing oxidative stress; furthermore, the protective effect of OPLE against renal damage in diabetes depends on the dose of OPLE as well as progression of DN. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38447/1/Chronic_Administration_of_Oil_Palm_%28Elaeis_guineensis%29_Leaves_Extract_Attenuates.pdf Rajavel, Varatharajan and Abdul Sattar, Munavvar Zubaid and Abdulla, Mahmood Ameen and M. Kassim, Normadiah and Abdullah, Nor Azizan (2012) Chronic Administration of Oil Palm (Elaeis guineensis ) Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative Stress and Improves Renal Histopathology and Function in Experimental Diabetes. Evidence-Based Complementary and Alternative Medicine, 2012 (195367). pp. 1-12. ISSN 1741-427X https://doi.org/10.1155/2012/195367 |
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RS1-441 Pharmacy and materia medica Rajavel, Varatharajan Abdul Sattar, Munavvar Zubaid Abdulla, Mahmood Ameen M. Kassim, Normadiah Abdullah, Nor Azizan Chronic Administration of Oil Palm (Elaeis guineensis ) Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative Stress and Improves Renal Histopathology and Function in Experimental Diabetes |
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Oil palm (Elaeis guineensis) leaves extract (OPLE) has antioxidant properties and because oxidative stress contributes to the
pathogenesis of diabetic nephropathy (DN), we tested the hypothesis that OPLE prevents diabetes renal oxidative stress,
attenuating injury. Sprague-Dawley rats received OPLE (200 and 500 mg kg−1) for 4 and 12 weeks after diabetes induction
(streptozotocin 60mg kg−1). Blood glucose level, body and kidney weights, urine flow rate (UFR), glomerular filtration rate
(GFR), and proteinuria were assessed. Oxidative stress variables such as 8-hydroxy-2�-deoxyguanosine (8-OHdG), glutathione
(GSH), and lipid peroxides (LPO) were quantified. Renalmorphology was analysed, and plasma transforming growth factor-beta1
(TGF-β1) was measured. Diabetic rats demonstrated increase in blood glucose and decreased body and increased kidney weights.
Renal dysfunction (proteinuria, elevations in UFR and GFR) was observed in association with increases in LPO, 8-OHdG, and
TGF-β1 and a decrease in GSH. Histological evaluation of diabetic kidney demonstrated glomerulosclerosis and tubulointerstitial
fibrosis. OPLE attenuated renal dysfunction, improved oxidative stress markers, and reduced renal pathology in diabetic animals.
These results suggest OPLE improves renal dysfunction and pathology in diabetes by reducing oxidative stress; furthermore, the
protective effect of OPLE against renal damage in diabetes depends on the dose of OPLE as well as progression of DN. |
format |
Article |
author |
Rajavel, Varatharajan Abdul Sattar, Munavvar Zubaid Abdulla, Mahmood Ameen M. Kassim, Normadiah Abdullah, Nor Azizan |
author_facet |
Rajavel, Varatharajan Abdul Sattar, Munavvar Zubaid Abdulla, Mahmood Ameen M. Kassim, Normadiah Abdullah, Nor Azizan |
author_sort |
Rajavel, Varatharajan |
title |
Chronic Administration of Oil Palm (Elaeis guineensis )
Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative
Stress and Improves Renal Histopathology and Function in
Experimental Diabetes |
title_short |
Chronic Administration of Oil Palm (Elaeis guineensis )
Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative
Stress and Improves Renal Histopathology and Function in
Experimental Diabetes |
title_full |
Chronic Administration of Oil Palm (Elaeis guineensis )
Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative
Stress and Improves Renal Histopathology and Function in
Experimental Diabetes |
title_fullStr |
Chronic Administration of Oil Palm (Elaeis guineensis )
Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative
Stress and Improves Renal Histopathology and Function in
Experimental Diabetes |
title_full_unstemmed |
Chronic Administration of Oil Palm (Elaeis guineensis )
Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative
Stress and Improves Renal Histopathology and Function in
Experimental Diabetes |
title_sort |
chronic administration of oil palm (elaeis guineensis )
leaves extract attenuates hyperglycaemic-induced oxidative
stress and improves renal histopathology and function in
experimental diabetes |
publisher |
Hindawi Publishing Corporation |
publishDate |
2012 |
url |
http://eprints.usm.my/38447/1/Chronic_Administration_of_Oil_Palm_%28Elaeis_guineensis%29_Leaves_Extract_Attenuates.pdf http://eprints.usm.my/38447/ https://doi.org/10.1155/2012/195367 |
_version_ |
1643709360168239104 |
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13.18916 |