Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morph...
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2012
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Online Access: | http://eprints.usm.my/38380/1/Autophagic_Cell_Death_Is_Induced_by_Acetone_and_Ethyl_Acetate_Extracts_from_Eupatorium_odoratum_In_Vitro.pdf http://eprints.usm.my/38380/ https://doi.org/10.1100/2012/439479 |
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my.usm.eprints.38380 http://eprints.usm.my/38380/ Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines Harun, Faizah Syed Sahil Jamalullail, Syed Mohsin Khoo, Boon Yin Othman, Zulkhairi Tilwari, Anita Balaram, Prabha R735-854 Medical education. Medical schools. Research Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity,membrane blebbing and ballooning at 24 hours, replacement bymass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts of EO induce cell death through induction of autophagy and hold potential for development as potential anticancer drugs. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38380/1/Autophagic_Cell_Death_Is_Induced_by_Acetone_and_Ethyl_Acetate_Extracts_from_Eupatorium_odoratum_In_Vitro.pdf Harun, Faizah and Syed Sahil Jamalullail, Syed Mohsin and Khoo, Boon Yin and Othman, Zulkhairi and Tilwari, Anita and Balaram, Prabha (2012) Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines. Scientific World Journal, 2012 (439479). pp. 1-9. ISSN 2356-6140 https://doi.org/10.1100/2012/439479 |
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R735-854 Medical education. Medical schools. Research Harun, Faizah Syed Sahil Jamalullail, Syed Mohsin Khoo, Boon Yin Othman, Zulkhairi Tilwari, Anita Balaram, Prabha Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines |
description |
Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented.
This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the
cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A
protein expression. Cytotoxic activity,membrane blebbing and ballooning at 24 hours, replacement bymass vacuolation, and double
membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA
fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M
arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone
and ethyl acetate extracts of EO induce cell death through induction of autophagy and hold potential for development as potential
anticancer drugs. |
format |
Article |
author |
Harun, Faizah Syed Sahil Jamalullail, Syed Mohsin Khoo, Boon Yin Othman, Zulkhairi Tilwari, Anita Balaram, Prabha |
author_facet |
Harun, Faizah Syed Sahil Jamalullail, Syed Mohsin Khoo, Boon Yin Othman, Zulkhairi Tilwari, Anita Balaram, Prabha |
author_sort |
Harun, Faizah |
title |
Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines |
title_short |
Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines |
title_full |
Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines |
title_fullStr |
Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines |
title_full_unstemmed |
Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines |
title_sort |
autophagic cell death is induced by acetone and ethyl acetate extracts fromeupatorium odoratum invitro : effects onmcf-7 and vero cell lines |
publisher |
Hindawi Publishing Corporation |
publishDate |
2012 |
url |
http://eprints.usm.my/38380/1/Autophagic_Cell_Death_Is_Induced_by_Acetone_and_Ethyl_Acetate_Extracts_from_Eupatorium_odoratum_In_Vitro.pdf http://eprints.usm.my/38380/ https://doi.org/10.1100/2012/439479 |
_version_ |
1643709340668919808 |
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13.18916 |