Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium
Objectives: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis sp...
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my.usm.eprints.36795 http://eprints.usm.my/36795/ Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium Saniasiaya, Jeyasakthy Salim, Rosdan Mohamad, Irfan Harun, Azian R5-130.5 General works Objectives: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans. Methods: This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC). Results: For A. niger, a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of Aloe vera leaf for C. albicans. The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone of inhibition was obtained for C. albicans the MIC was not determined. Conclusions: The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the aqueous extract for A. niger (p < 0.001). Malaysian Aloe vera has a significant antifungal effect towards A. niger Oman Medical Specialty Board 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/36795/1/%28Antifungal_Effect_of_Malaysian%29_OS-OMJ-D-16-00061_%2805J%29.pdf Saniasiaya, Jeyasakthy and Salim, Rosdan and Mohamad, Irfan and Harun, Azian (2017) Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium. Oman Medical Journal, 32 (1). pp. 41-46. ISSN 1999-768X https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187399/ |
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R5-130.5 General works Saniasiaya, Jeyasakthy Salim, Rosdan Mohamad, Irfan Harun, Azian Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium |
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Objectives: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes
since ancient times with antifungal activity known to be amongst its medicinal properties.
We conducted a pilot study to determine the antifungal properties of Malaysian Aloe
vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans.
Methods: This laboratory-controlled prospective study was conducted at the Universiti
Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions
via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal
isolates were inoculated with the five different concentrations of each extract (50 g/mL,
25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method.
Zone of inhibition was measured followed by minimum inhibitory concentration
(MIC). Results: For A. niger, a zone of inhibition for alcohol and aqueous extract
was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for
both alcohol and aqueous extracts of Aloe vera leaf for C. albicans. The MIC values of
aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone
of inhibition was obtained for C. albicans the MIC was not determined. Conclusions:
The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the
aqueous extract for A. niger (p < 0.001). Malaysian Aloe vera has a significant antifungal
effect towards A. niger |
format |
Article |
author |
Saniasiaya, Jeyasakthy Salim, Rosdan Mohamad, Irfan Harun, Azian |
author_facet |
Saniasiaya, Jeyasakthy Salim, Rosdan Mohamad, Irfan Harun, Azian |
author_sort |
Saniasiaya, Jeyasakthy |
title |
Antifungal Effect of Malaysian Aloe vera Leaf
Extract on Selected Fungal Species of Pathogenic
Otomycosis Species in In Vitro Culture Medium |
title_short |
Antifungal Effect of Malaysian Aloe vera Leaf
Extract on Selected Fungal Species of Pathogenic
Otomycosis Species in In Vitro Culture Medium |
title_full |
Antifungal Effect of Malaysian Aloe vera Leaf
Extract on Selected Fungal Species of Pathogenic
Otomycosis Species in In Vitro Culture Medium |
title_fullStr |
Antifungal Effect of Malaysian Aloe vera Leaf
Extract on Selected Fungal Species of Pathogenic
Otomycosis Species in In Vitro Culture Medium |
title_full_unstemmed |
Antifungal Effect of Malaysian Aloe vera Leaf
Extract on Selected Fungal Species of Pathogenic
Otomycosis Species in In Vitro Culture Medium |
title_sort |
antifungal effect of malaysian aloe vera leaf
extract on selected fungal species of pathogenic
otomycosis species in in vitro culture medium |
publisher |
Oman Medical Specialty Board |
publishDate |
2017 |
url |
http://eprints.usm.my/36795/1/%28Antifungal_Effect_of_Malaysian%29_OS-OMJ-D-16-00061_%2805J%29.pdf http://eprints.usm.my/36795/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187399/ |
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1643708887968251904 |
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13.15806 |