Pengekspresian dan kajian imunologi ke atas gen mtp40 daripada Mycobacterium tuberculosis

• Main Author: Ismail, Aziah
• Format: Thesis
• Language: English
• Published: 2001
• Subjects: R5-130.5 General works
• Online Access: http://eprints.usm.my/36076/1/aziah_ismail-mrm_%28NN1%2924.pdf; http://eprints.usm.my/36076/

Pembangunan vaksin yang berkesan adalah dianggap satu kaedah utama dalam kawalan terhadap tuberkulosis di seluruh dunia. Fokus utama projek ini ialah protein MTP40 pada M. tuberculosis yang telah dilaporkan sebelum ini mengandungi keduadua epitop sel B dan sel T. Dalam kajian ini gerak balas keimunan terhadap 7 peptida yang diterbitkan daripada protein MTP40 dalam M. tuberculosis telah diuji dengan kaedah "enzyme linked immunosorbent assay" (ELISA) dan "lymphoblastic transformation test" (L TT) menggunakan spesimen pesakit TB (TB}, individu yang terdedah kepada TB tanpa gejala (HC) dan individu kawalan (NC). Gerak balas antibodi terhadap peptida MTP40 secara umumnya didapati lebih tinggi dalam kumpulan TB berbanding dengan kumpulan HC tetapi hanya peptida P1 dan P2 yang memberikan perbezaan yang signifikan pada kedua-dua kumpulan tersebut. Di samping itu, gerak balas limfosit terhadap peptida tersebut adalah lebih tinggi di kalangan individu kumpulan HC berbanding dengan kumpulan TB di mana P1, P2, PS dan P6 memberikan perbezaan yang signifikan. Keputusan ini mencadangkan MTP40 mungkin bergunak sebagai calon vaksin. Kajian lanjut telah dilakukan dengan lebih mendalam untuk mengkaji kemungkinan ini. The development of improved vaccines is considered a high priority in the effort to control tuberculosis worldwide. The focus of this project is the MTP40 protein of Mycobacterium tuberculosis which have been previously reported to contain both 8 and T cell epitopes. In this study, immune response to 7 peptides (P1-P7) derived from the MTP40 protein of M. tuberculosis were tested by enzyme linked immunosorbent assay (ELISA) and lymphoblastic transformation test (L TT) using specimens from tuberculosis patients (TB), healthy contacts (HC) and normal controls (NC). Antibody response to MTP40 peptides were generally found to be higher in the TB group as compared to the HC group but only in peptides P1 (p<0.005) and P2 (p<0.01) were the difference between the two groups found to be statistically significant. On the other hand, lymphocyte responses to these peptides were generally found to be higher in the HC group as compared to the TB group with P1, P2, P5 and P6 showing statistically significant differences. This results suggest that MTP40 may be useful as a vaccine candidate. Further work was done to explore this possibility.