Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts

• Main Author: Mohamad, Nurul Najwa
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: RS1-441 Pharmacy and materia medica
• Online Access: http://eprints.usm.my/32243/1/NURUL_NAJWA_MOHAMAD_24%28NN%29.pdf; http://eprints.usm.my/32243/

Piper betle L. telah digunakan secara meluas dalam perubatan herba tradisional di Asia. Walau bagaimanapun, tumbuhan ini masih kekurangan data analisis terutama berkaitan kualiti, keselamatan dan keberkesanannya. Kajian ini telah dijalankan untuk menyediakan maklumat mengenai pemiawaian daun Piper betle. Kajian ini dibahagikan kepada tiga bahagian: kualiti dan keselamatan bahan-bahan mentah daun Piper betle, piawaian ekstrak Piper betle menggunakan sebatian terasing dan anti-obesiti. Daun kering Piper betle ditentukan untuk kandungan logam berat dan kandungan ujian had mikrob (MLT). Tumbuhan ini didapati berada dalam lingkungan had yang ditetapkan bagi empat logam berat Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) dan ia bebas dari pencemaran mikrob. Pemeriksaan fitokimia awal bagi ekstrak etanol, ekstrak 50% etanol dan ekstrak air menunjukkan kehadiran sebatian terpenoid, flavonoid, fenol, sterol, koumarin dan saponin. Dua sebatian iaitu kavibetol (CHV) dan hidroksikavikol (HC) telah diasingkan dan dikenalpasti melalui data UV, FTIR, TLC, HPLC, GC-MS dan NMR. Profil kimia yang menggunakan teknik spektroskopi dan kromatografi seperti UV, FTIR, HPTLC dan GC-MS digunakan dalam kajian pemiawaian. Piper betle L. has been extensively used in traditional herbal remedies in Asia. Nevertheless, this plant still lacks of data especially on their quality, safety and efficacy. This study has been conducted to provide information on the standardization of Piper betle leaves. The study was divided into three parts: quality and safety of raw materials of Piper betle leaves, standardization of Piper betle extracts using isolation compounds and anti-obesity studies. Dry powdered leaves of Piper betle were determined to evaluate the content of heavy metals and the microbial limit test (MLT). This plant was found to be in the range of the acceptable limit of four main heavy metals Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) and free from microbial contamination. The preliminary phytochemical screening using TLC on the ethanol, 50% ethanol and water extracts showed the presence of terpenoids, flavonoids, phenols, sterols, coumarins and saponins. Two compounds namely chavibetol (CHV) and hydroxychavicol (HC) were isolated and identified by UV, FTIR, HPTLC, HPLC, GC-MS and NMR data. The chemical profile using spectroscopic and chromatographic techniques such as UV, FTIR, HPTLC and GC-MS were used in the standardization.