Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor
Polihidroksialkanoat (PHA) merupakan termoplastik bakteria yang terbiodegradasi secara semulajadi. Kopolimer poli(3-hidroksibutirat-ko-4-hidroksibutirat) P(3HB-ko-4HB) merupakan sejenis PHA yang dikenali dengan sifat kebioserasian yang dapat ditransformasikan sebagai produk biofarmaseutikal dan digu...
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my.usm.eprints.32178 http://eprints.usm.my/32178/ Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor Ku Abdul Rahamn, Ku Nur Elina Shaheeda QH1 Natural history (General - Including nature conservation, geographical distribution) Polihidroksialkanoat (PHA) merupakan termoplastik bakteria yang terbiodegradasi secara semulajadi. Kopolimer poli(3-hidroksibutirat-ko-4-hidroksibutirat) P(3HB-ko-4HB) merupakan sejenis PHA yang dikenali dengan sifat kebioserasian yang dapat ditransformasikan sebagai produk biofarmaseutikal dan digunakan dalam bidang perubatan. Berat molekul yang tinggi (>1MDa) adalah sifat yang diidamkan kerana ia berpotensi untuk pelbagai aplikasi penggunaan memandangkan 1,8-oktanadiol dapat menghasilkan 4HB monomer dengan komposisi yang tinggi tetapi mempunyai kepekatan PHA yang rendah. Kajian ini dijalankan untuk mengkaji potensi 1,8-oktanadiol untuk menghasilkan P(3HB-ko-4HB) dengan berat molekul yang tinggi serta kepekatan PHA yang lebih baik menggunakan kultur substrat campuran. Proses pengkulturan satu peringkat telah dilakukan dengan menggabungkan karbon 1,8-oktanadiol dengan asid oleik, γ-butirolakton, 1,4-butanadiol atau natrium 4-hidroksibutirat. Kesan nisbah sumber karbon kepada nitrogen (C/N) oleh 1,8-oktanadiol telah dikaji dan menunjukkan C/N=10 memberikan pertumbuhan dan kepekatan PHA yang optimum. Kajian pengkulturan substrat campuran ke atas biosintesis kopolimer menggunakan kepekatan dan substrat campuran yang berbeza melalui kajian kelalang goncangan menghasilkan 4 hingga 52 mol% komposisi 4HB monomer oleh kultur sesekelompok dan kultur suapan sesekelompok. Polyhydroxyalkanoate (PHA) is a bacterial derived thermoplastic that is naturally biodegradable. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is a PHA known for its biocompatibility that can be transformed into biopharmaceutical products and be used in medical applications. High molecular weight polymer (>1 MDa) is highly desired for its many potential applications usage as 1,8-octanediol could produce high 4HB composition but low PHA concentration. This study investigates the potential of 1,8-octanediol to produce high molecular weight P(3HB-co-4HB) copolymer with improved PHA concentration using mixed substrate cultivation. One-stage cultivation process was performed using different carbon combination of 1,8-octanediol either with oleic acid, γ-butyrolactone, 1,4-butanediol or sodium 4-hydroxybutyrate. The effect of carbon to nitrogen (C/N) ratios of 1,8-octanediol was investigated and it was found that C/N=10 gave the optimum growth and PHA concentration. Biosynthesis of copolymer using different concentration of mixed-substrate by shake-flask cultivation resulted in 4 to 52 mol% 4HB monomer compositions by batch and fed-batch fermentation. 2016-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/32178/1/KU_NUR_ELINA_SHAHEEDA_KU_ABDUL_RAHMAN_24%28NN%29.pdf Ku Abdul Rahamn, Ku Nur Elina Shaheeda (2016) Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor. Masters thesis, Universiti Sains Malaysia. |
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QH1 Natural history (General - Including nature conservation, geographical distribution) Ku Abdul Rahamn, Ku Nur Elina Shaheeda Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor |
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Polihidroksialkanoat (PHA) merupakan termoplastik bakteria yang terbiodegradasi secara semulajadi. Kopolimer poli(3-hidroksibutirat-ko-4-hidroksibutirat) P(3HB-ko-4HB) merupakan sejenis PHA yang dikenali dengan sifat kebioserasian yang dapat ditransformasikan sebagai produk biofarmaseutikal dan digunakan dalam bidang perubatan. Berat molekul yang tinggi (>1MDa) adalah sifat yang diidamkan kerana ia berpotensi untuk pelbagai aplikasi penggunaan memandangkan 1,8-oktanadiol dapat menghasilkan 4HB monomer dengan komposisi yang tinggi tetapi mempunyai kepekatan PHA yang rendah. Kajian ini dijalankan untuk mengkaji potensi 1,8-oktanadiol untuk menghasilkan P(3HB-ko-4HB) dengan berat molekul yang tinggi serta kepekatan PHA yang lebih baik menggunakan kultur substrat campuran. Proses pengkulturan satu peringkat telah dilakukan dengan menggabungkan karbon 1,8-oktanadiol dengan asid oleik, γ-butirolakton, 1,4-butanadiol atau natrium 4-hidroksibutirat. Kesan nisbah sumber karbon kepada nitrogen (C/N) oleh 1,8-oktanadiol telah dikaji dan menunjukkan C/N=10 memberikan pertumbuhan dan kepekatan PHA yang optimum. Kajian pengkulturan substrat campuran ke atas biosintesis kopolimer menggunakan kepekatan dan substrat campuran yang berbeza melalui kajian kelalang goncangan menghasilkan 4 hingga 52 mol% komposisi 4HB monomer oleh kultur sesekelompok dan kultur suapan sesekelompok.
Polyhydroxyalkanoate (PHA) is a bacterial derived thermoplastic that is naturally biodegradable. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is a PHA known for its biocompatibility that can be transformed into biopharmaceutical products and be used in medical applications. High molecular weight polymer (>1 MDa) is highly desired for its many potential applications usage as 1,8-octanediol could produce high 4HB composition but low PHA concentration. This study investigates the potential of 1,8-octanediol to produce high molecular weight P(3HB-co-4HB) copolymer with improved PHA concentration using mixed substrate cultivation. One-stage cultivation process was performed using different carbon combination of 1,8-octanediol either with oleic acid, γ-butyrolactone, 1,4-butanediol or sodium 4-hydroxybutyrate. The effect of carbon to nitrogen (C/N) ratios of 1,8-octanediol was investigated and it was found that C/N=10 gave the optimum growth and PHA concentration. Biosynthesis of copolymer using different concentration of mixed-substrate by shake-flask cultivation resulted in 4 to 52 mol% 4HB monomer compositions by batch and fed-batch fermentation.
|
format |
Thesis |
author |
Ku Abdul Rahamn, Ku Nur Elina Shaheeda |
author_facet |
Ku Abdul Rahamn, Ku Nur Elina Shaheeda |
author_sort |
Ku Abdul Rahamn, Ku Nur Elina Shaheeda |
title |
Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor |
title_short |
Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor |
title_full |
Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor |
title_fullStr |
Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor |
title_full_unstemmed |
Biosynthesis Of High Molecular Weight Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer By Cupriavidus Sp. USMAA1020 Using 1,8-Octanediol As The Carbon Precursor |
title_sort |
biosynthesis of high molecular weight poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer by cupriavidus sp. usmaa1020 using 1,8-octanediol as the carbon precursor |
publishDate |
2016 |
url |
http://eprints.usm.my/32178/1/KU_NUR_ELINA_SHAHEEDA_KU_ABDUL_RAHMAN_24%28NN%29.pdf http://eprints.usm.my/32178/ |
_version_ |
1643707583439044608 |
score |
13.18916 |