Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro

• Main Author: Leong, Yin Yee
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: RK1-715 Dentistry
• Online Access: http://eprints.usm.my/32076/1/LEONG_YIN_YEE_24%28NN%29.pdf; http://eprints.usm.my/32076/

Latar Belakang: Sel-sel tunjang mesenkima (MSCs) terbukti dalam membantu proses pembaikan jantung melalui pengaktifan sel-sel jantung (CSCs) berikutan infaksi, tetapi pengeksploitasian secara in vitro melibatkan sinergi antara dua sel sebelum pemindahan sel masih kurang diketahui. Objektif: Kajian ini bertujuan untuk mengkaji kesan sinergi MSCs kepada diferensiasi sel kardiomiosit. Kaedah: CSCs yang mengekspres c-kit dan MSCs tulang diperoleh daripada mencit C57BL/6N (n=9). Diferensiasi CSCs diuji selepas diasingkan daripada MSCs berikutan ko-kultur dengan menggunakan zarah oksida besi bersaiz micron, dan dibandingkan dengan CSCs yang dirawat dengan medium terkondisi MSCs atau tanpa sebarang rawatan. Diferensiasi kardiomiosit dikesan menggunakan penwarnaan imunofluorescein dan diukur menggunakan tindakbalas polimerase berantai secara quantitatif. Semua data dianalis dengan ANOVA sehala. Background: Bone marrow-derived mesenchymal stem cells (MSCs) has been shown to facilitate heart repair via activation of endogenous cardiac stem cells (CSCs) following infarction, but little is known if the synergy of the two cells can be exploited in vitro prior to transplantation. Objective: This study aimed to examine the synergistic effects of MSCs on CSC cardiomyocyte differentiation. Methods: C-kit CSCs and MSCs were isolated from 4-6 weeks C57BL/6N mice (n=9). CSC cardiomyocyte differentiation was tested after re-isolated from MSC co-culture using micron-sized particles of iron oxide, and compared to CSCs treated with MSC-conditioned medium (CdM) or without any treatment. Cardiomyocyte differentiation was detected using immunofluorescence staining and quantified using qPCR. All data were analysed by one way ANOVA.