Reduction Of Escherichia Coli In Ablution, Lake And River Water Using Porcelanite

• Main Author: Salih, Suhair Shatti
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: T1-995 Technology(General)
• Online Access: http://eprints.usm.my/32031/1/SUHAIR_SHATTI_SALIH_24%28NN%29.pdf; http://eprints.usm.my/32031/

Kajian ini bertujuan untuk menyiasat penggunaan porselanit dan klorin (natrium hipoklorit) untuk pengurangan E.coli dalam sampel air yang diperolehi dari Sungai Pinang, Tasik Harapan, dan air wuduk dari Masjid Khalid, Universiti Sains Malaysia, Pulau Pinang. Kesan pH, dos porselanit, saiz butiran dan suhu telah dinilai. Eksperimen ini telah dijalankan ke atas pelbagai jenis dos (40, 50, 60, 70, 80, dan 90 mg/ L), saiz butiran porselanit (0.09, 0.1, 0.2, 0.3, 0.4, dan 0.5 mm), pH (5, 6, 7, 8, dan 9) dan suhu (10, 20, 30, dan 40℃). Proses makmal yang terlibat ialah pengenceran sampel (1:10 dan 1:100) dan inokulasi pada agar MacConkey dan pada petri agar Eosin Metilena Biru. Selepas pengeraman, koloni-koloni telah dihitung menggunakan pengira koloni. Keputusan yang diperolehi daripada 108-larian (dengan tiga replikasi) reka bentuk faktorial telah dianalisis. Analisis varians (ANOVA) mendedahkan bahawa model disebut melengkapi secukupnya data ujikaji bagi semua tindak balas. This study aimed to investigate the use of porcelanite and chlorine (sodium hypochlorite) for the reduction E.coli in water samples obtained from Pinang River, Harapan Lake, and ablution water from Khalid Mosque, Universiti Sains Malaysia, Pulau Pinang. The effects of pH, dosage of porcelanite, grain size and temperature were evaluated. The experiments were performed over a wide range of dosage (40, 50, 60, 70, 80, and 90 mg/L), porcelanite grain size (0.09, 0.1, 0.2, 0.3, 0.4, and 0.5 mm), pH (5, 6, 7, 8, and 9) and temperature (10, 20, 30, and 40 ). The laboratory process involved in this study was preparation of serial dilutions of the sample (1:10 and 1:100) and cultivating these dilutions on MacConkey agar and Eosin Methylene Blue agar plate. After incubation, the colonies were counted using colony counter. The results obtained from 108-runs (with three replicates) factorial design were analyzed. The analysis of variance (ANOVA) revealed that the mentioned model adequately fitted the experimental data for all responses.