Anti-Tumor Study Of Scopoletin And Rubbing-Mercapto-Nitrile From Nicotiana Glauca

• Main Author: Mahfooth, Yasser Tabana
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: RS1-441 Pharmacy and materia medica
• Online Access: http://eprints.usm.my/31884/1/YASSER_TABANA_24%28NN%29.pdf; http://eprints.usm.my/31884/

Nicotiana glauca (Solanaceae) telah digunakan secara tradisional untuk merawat jangkitan dan kanser. Tumbuhan ini dilaporkan kaya dengan komponen–komponen yang baik untuk kesihatan. Dalam kajian ini, dua komponen anti-tumor daripada ekstrak n-heksana N. glauca telah berjaya diasingkan, iaitu scopoletin dan satu komponen baru, Rubbing-Mercapto-Nitrile (RMN). Pengasingan scopoletin berpandukan aktiviti anti-angiogenesis telah dijalankan menggunakan ekstrak daripada batang N. glauca. Aktiviti anti-angiogenesis scopoletin dikaji menggunakan model–model angiogenesis secara ex vivo dan in vivo. Keberkesanan anti-tumorigenik scopoletin dikaji menggunakan xenograf tumor kolorektal manusia pada mencit nude atimik. Scopoletin menyebabkan perencatan signifikan dalam percambahan salur mikro pada eksplan aortik tikus dengan nilai kepekatan perencatan 50% adalah 34 μg/ml. Scopoletin (100 dan 200 mg/kg) merencat dengan berkesan vaskularisasi plak matrigel yang diimplan pada mencit nude, masing–masing sebanyak 59.72% dan 89.4%. Nicotiana glauca (Solanaceae) has been traditionally used as a folk remedy to treat infections and cancer. It has been reported as a rich source of beneficial phytochemicals. In the present study, two anti-tumor compounds derived from n-hexane extract of N. glauca namely scopoletin and, a novel compound, Rubbing-Mercapto-Nitrile (RMN) have been isolated. Antiangiogenesis-guided isolation of scopoletin was conducted using an extract from the stem of N. glauca. The anti-angiogenic activity of scopoletin was investigated using ex vivo and in vivo angiogenesis models. The antitumorigenic efficacy of scopoletin was studied in human colorectal tumor xenografts using athymic nude mice. Scopoletin caused significant suppression of microvessel sprouting in rat aortic explants with IC50 of 34 μg/ml. Scopoletin (100 and 200 mg/kg) strongly inhibited (59.72% and 89.4%, respectively) vascularization in matrigel plugs implanted in nude mice.