Distribution Of Lachnum, Pteridocolous Fungi On Cyathea In Peninsular Malaysia

• Main Author: Mohd Razikin, Muhammad Zulfa
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/31877/1/MUHAMMAD_ZULFA_BIN_MOHD_RAZIKIN_24%28NN%29.pdf; http://eprints.usm.my/31877/

Kulat pteridokolus hidup dan berkembang terutamanya pada batang yang mati atau reput daripada pakis dan sekutu pakis. Spesies pokok pakis di Malaysia telah tersebar secara meluas di tanah rendah dan menyesuaikan diri dengan persekitaran tanah tinggi. Dalam kajian ini, sampel kulat pteridokolus telah dikutip dari Bukit Bendera, Bukit Larut, Cameron Highlands dan Gunung Ledang. Berdasarkan ciri-ciri morfologi, empat spesies Lachnum iaitu L. oncospermatum, L. lanariceps, Lachnum sp. 1 dan Lachnum sp. 2 telah dikenalpasti dengan membezakan bentuk apotesium, warna bahan resin pada apotesium, dan saiz askospora. Dalam kajian molekul, sembilan pencilan telah digunakan untuk analisis filogenetik. Jujukan DNA ITS-5.8S rDNA, rantau D1-D2 rDNA subunit yang besar, dan gabungan data ITS-5.8S dan D1-D2 dianalisa. Pokok filogenetik telah dibina dengan menggunakan kaedah sambungan jiran (NJ) dan parsimoni maksimum (MP). Pteridocolous fungi are living and growing particularly on dead or decayed rachis of pteridophyte. Tree fern species in Malaysia are widely distributed in the lowland and adapted to the highland environment. In this study, samples of pteridocolous fungi were collected from Bukit Bendera, Bukit Larut, Cameron Highlands and Gunung Ledang. Based on the morphological characteristics, four Lachnum species namely L. oncospermatum, L. lanariceps, Lachnum sp. 1 and Lachnum sp. 2 were identified by distinguishing the shape of the apothecium, colour of resinous matter of the apothecium and the size of ascospores. In molecular study, 9 isolates were used for phylogenetic analyses. The DNA sequences of ITS-5.8S rDNA, the D1-D2 region of the large subunit rDNA, and combined data of ITS-5.8S and D1-D2 were analyzed. Phylogenetic trees were constructed by using Neighbour-Joining (NJ) and Maximum-Parsimony (MP) methods.