Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King

Keperluan untuk mendapatkan pigmen semula jadi daripada sumber mikrob telah berkembang dengan pesatnya memandangkan kebanyakam pigmen sintetik didapati merbahaya kepada manusia. Namun begitu, banyak pigmen semula jadi daripada sumber alamiah yang lain adalah tidak mencukupi untuk memenuhi keperluan...

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Main Author: Iu , Chai Woei
Format: Thesis
Language:English
Published: 2016
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Online Access:http://eprints.usm.my/31531/1/IU_CHAI_WOEI_24.pdf
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spelling my.usm.eprints.31531 http://eprints.usm.my/31531/ Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King Iu , Chai Woei QH1 Natural history (General - Including nature conservation, geographical distribution) Keperluan untuk mendapatkan pigmen semula jadi daripada sumber mikrob telah berkembang dengan pesatnya memandangkan kebanyakam pigmen sintetik didapati merbahaya kepada manusia. Namun begitu, banyak pigmen semula jadi daripada sumber alamiah yang lain adalah tidak mencukupi untuk memenuhi keperluan industri. Dalam penyelidikan ini, enam pencilan kulat endofitik yang dipencilkan daripada daun Swietenia macrophylla telah dipilih sebagai subjek kajian tahap awal dan Nigrospora sphaerica CL – OP 30 telah dipilih sebagai sasaran tunggal kajian ini disebabkan oleh keupayaan kulat tersebut menghasilkan pigmen merah yang paling tinggi pada hari ke-14 dengan hasilan sebanyak 0.1471 OD/g berbanding dengan pencilan lain. Kulat tersebut didapati menghasilkan lebih banyak pigmen merah dalam keadaan pengkulturan bercahaya tanpa kehadiran ekstrak tumbuhan perumahnya dan saiz inoculum terbaik adalah dengan kehadiran dua plag kulat berdiameter 1 cm. The demand for natural pigment from microbial sources are growing rapidly since most of the synthetic pigments are found to be hazardous to mankind, and most of the natural pigments obtained from other natural sources are insufficient for the industrial purposes. In this study, six endophytic fungal isolates which were isolated from Swietenia macrophylla were selected as the preliminary study subjects where Nigrospora sphaerica CL-OP 30 was eventually selected as the sole target for the study as the fungus produced highest yield of reddish pigment at day 14 with the 0.1471 OD/g of production compared to others. The higher pigmentation was favourable when the fungus was cultivated in light condition without the incorporation of host plant extract with the ideal inoculum size of the fungus, 2 fungal plugs with 1cm in diameter. 2016-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31531/1/IU_CHAI_WOEI_24.pdf Iu , Chai Woei (2016) Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Iu , Chai Woei
Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
description Keperluan untuk mendapatkan pigmen semula jadi daripada sumber mikrob telah berkembang dengan pesatnya memandangkan kebanyakam pigmen sintetik didapati merbahaya kepada manusia. Namun begitu, banyak pigmen semula jadi daripada sumber alamiah yang lain adalah tidak mencukupi untuk memenuhi keperluan industri. Dalam penyelidikan ini, enam pencilan kulat endofitik yang dipencilkan daripada daun Swietenia macrophylla telah dipilih sebagai subjek kajian tahap awal dan Nigrospora sphaerica CL – OP 30 telah dipilih sebagai sasaran tunggal kajian ini disebabkan oleh keupayaan kulat tersebut menghasilkan pigmen merah yang paling tinggi pada hari ke-14 dengan hasilan sebanyak 0.1471 OD/g berbanding dengan pencilan lain. Kulat tersebut didapati menghasilkan lebih banyak pigmen merah dalam keadaan pengkulturan bercahaya tanpa kehadiran ekstrak tumbuhan perumahnya dan saiz inoculum terbaik adalah dengan kehadiran dua plag kulat berdiameter 1 cm. The demand for natural pigment from microbial sources are growing rapidly since most of the synthetic pigments are found to be hazardous to mankind, and most of the natural pigments obtained from other natural sources are insufficient for the industrial purposes. In this study, six endophytic fungal isolates which were isolated from Swietenia macrophylla were selected as the preliminary study subjects where Nigrospora sphaerica CL-OP 30 was eventually selected as the sole target for the study as the fungus produced highest yield of reddish pigment at day 14 with the 0.1471 OD/g of production compared to others. The higher pigmentation was favourable when the fungus was cultivated in light condition without the incorporation of host plant extract with the ideal inoculum size of the fungus, 2 fungal plugs with 1cm in diameter.
format Thesis
author Iu , Chai Woei
author_facet Iu , Chai Woei
author_sort Iu , Chai Woei
title Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_short Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_full Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_fullStr Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_full_unstemmed Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_sort natural red pigment with antibacterial activity from nigrospora sphaerica cl-op 30, an endophytic fungus isolated from leaf of swietenia macrophylla king
publishDate 2016
url http://eprints.usm.my/31531/1/IU_CHAI_WOEI_24.pdf
http://eprints.usm.my/31531/
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score 13.18916