Feeding Strategies In Recombinant Human Erythropoietin Production By Pichia Pastoris Fermentation In Stirred-Tank Bioreactor

• Main Author: Shahkillahwati , Mohd Ridhwan
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/29011/1/FEEDING_STRATEGIES_IN_RECOMBINANT_HUMAN_ERYTHROPOIETIN_PRODUCTION_BY_pichia_pastoris_FERMENTATION_IN_STIRRED-TANK_BIOREACTOR.pdf; http://eprints.usm.my/29011/

Administration of erythropoietin or EPO as a therapeutic improves the quality of life in patients with cancer, renal and anemia. Knowing the importance of EPO and the high demand in the biopharmaceutical market, Pichia pastoris strains GS115 2 (5) has been genetically engineered to produce recombinant human erythropoietin (rhuEPO) from the synthetic gene using three-stage cultivation process. Pengunaan erythropietin atau EPO sebagai terapeutik dapat meningkatkan kualiti hidup di kalangan pesakit kanser, buah pinggang dan anemia. Mengetahui kepentingan EPO dan permintaan yang tinggi di pasaran biofarmaseutikal, rekombinan erythropoietin manusia (rhuEPO) telah dihasilkan secara genetik menggunakan Pichia pastoris strain GS115 2 (5) sebagai sel perumah melalui proses pengkulturan tiga-peringkat.