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Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants

Thesis (PhD)-- Faculty of Science and Technology, Universiti Sains Islam Malaysia, 2012

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Main Author: Asma Saleh W. Elmbrok
Format: Thesis
Language:English
Published: Universiti Sains Islam Malaysia 2015
Subjects:
Lactic acid bacteria
Online Access:http://ddms.usim.edu.my/handle/123456789/8828
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spelling my.usim-88282018-04-03T02:26:15Z Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants Asma Saleh W. Elmbrok Lactic acid bacteria Thesis (PhD)-- Faculty of Science and Technology, Universiti Sains Islam Malaysia, 2012 Chilli plants are easily attacked by fungi Colletotrichum resulting in great loss of this agricultural produce. This study evaluated the ability of lactic acid bacteria (LAB) as possible bio-control against fungi. Three hundred twenty four LABs were isolated from different sources in Malaysia and were evaluated for antifungal activity against C. capsici and C. gloeosporioides. The two isolates with good activity against both fungi were identified using API 50CHL and 16S rDNA as Lactobacillus plantarum LAB-C5 and Lactobacillus pentosus LAB-G7. Seeds treated with cell LAB-C5 showed significantly (P˂ 0.05) good germination percentage and growth of plumules and radicals compared to supernatant LAB-G7. The effect of chilli plants with LAB-C5 cells and C. capsici was evaluated. All chilli varieties showed increase in shoot and root dry weight with improved plant vigor. The biochemical analysis showed an increase in the total phenol compounds in fruits (with mean 2.71mg/g than control 1.83 mg/g). Increase in activity of enzymes peroxidase (PO) and polyphenoloxidase (PPO) in leaves was noted in plants treated with LAB-C5 cell. The PO was175.54 and PPO was 172.47 unit /enzyme of plant tissue, higher than control with PO of 89.86 and PPO of 97.27 unit/enzyme. An increase in lignin content (LC) of roots was 0.799 mg/g compared with control (0.329 mg/g). The cells LAB-C5 and supernatants LAB-G7 were effective as bio-control in vitro against fungal infection on red and green chilli obtained from markets and Cilibangi fruits during storage for 45 days at 28oC. Inoculation of LAB was able to generate resistance to fungal infection against both fungi in seeds, fruits and plants. Changes in biochemical compounds in chilli plants are factors that may contribute to the development of resistance in chilli varieties as shown by plants treated with LAB-C5 cell. The potential of using L. plantarum LAB-C5 as bio-control against C. capsici in chilli plants is possible and may reduce the use of chemical fungicides in the future. 2015-07-22T07:56:42Z 2015-07-22T07:56:42Z 2014 Thesis http://ddms.usim.edu.my/handle/123456789/8828 en Universiti Sains Islam Malaysia
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Lactic acid bacteria
spellingShingle Lactic acid bacteria
Asma Saleh W. Elmbrok
Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants
description Thesis (PhD)-- Faculty of Science and Technology, Universiti Sains Islam Malaysia, 2012
format Thesis
author Asma Saleh W. Elmbrok
author_facet Asma Saleh W. Elmbrok
author_sort Asma Saleh W. Elmbrok
title Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants
title_short Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants
title_full Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants
title_fullStr Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants
title_full_unstemmed Lactic Acid Bacteria As Biological Control Against Colletotrichum Capsici And Colletotrichum Gloeosporioides On Chilli Plants
title_sort lactic acid bacteria as biological control against colletotrichum capsici and colletotrichum gloeosporioides on chilli plants
publisher Universiti Sains Islam Malaysia
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8828
_version_ 1645152482302623744
score 13.222552

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