An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water

An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The curr...

Full description

Saved in:
Bibliographic Details
Main Authors: T., Ling Ling,, M., Ahmad,, L., Yook Heng,
Format: Article
Language:en_US
Published: 2015
Online Access:http://ddms.usim.edu.my/handle/123456789/8507
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usim-8507
record_format dspace
spelling my.usim-85072017-02-23T04:45:47Z An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water T., Ling Ling, M., Ahmad, L., Yook Heng, An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH 4 + ion whilst enzymatic amination of AlaDH and pyruvate is taking place. The biosensor was operated amperometrically at a potential of +0.6V and optimum pH 7. The NH 4 + biosensor demonstrated linear response to NH 4 + ion concentration in the range of 0.031.02mg/L with a limit of detection (LOD) of 8.52g/L. The proposed method has been successfully applied to the determination of NH 4 + ion in river water samples without any pretreatment. The levels of possible interferents in the waters were negligible to cause any interference on the proposed method. The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level. Copyright © 2011 Tan Ling Ling et al. 2015-06-25T06:57:10Z 2015-06-25T06:57:10Z 2011 Article 1687725X http://ddms.usim.edu.my/handle/123456789/8507 en_US
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
description An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH 4 + ion whilst enzymatic amination of AlaDH and pyruvate is taking place. The biosensor was operated amperometrically at a potential of +0.6V and optimum pH 7. The NH 4 + biosensor demonstrated linear response to NH 4 + ion concentration in the range of 0.031.02mg/L with a limit of detection (LOD) of 8.52g/L. The proposed method has been successfully applied to the determination of NH 4 + ion in river water samples without any pretreatment. The levels of possible interferents in the waters were negligible to cause any interference on the proposed method. The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level. Copyright © 2011 Tan Ling Ling et al.
format Article
author T., Ling Ling,
M., Ahmad,
L., Yook Heng,
spellingShingle T., Ling Ling,
M., Ahmad,
L., Yook Heng,
An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
author_facet T., Ling Ling,
M., Ahmad,
L., Yook Heng,
author_sort T., Ling Ling,
title An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
title_short An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
title_full An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
title_fullStr An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
title_full_unstemmed An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
title_sort amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8507
_version_ 1645152430509260800
score 13.214268