Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1
Strategic feeding of ammonium and metal ions (Mg2+, Mn2+, Fe3+, Cu2+, Ca2+, Co2+, and Zn2+) for enhanced GLA-rich lipid accumulation in C. bainieri 2A1 was established. When cultivated in nitrogen-limitedmedium, the fungus produced up to 30% lipid (g/g biomass) with 12.9% (g/g lipid) GLA. However, t...
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my.usim-84292017-02-23T04:43:22Z Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 Shuwahida, Shuib, Wan Nazatul Naziah, Wan Nawi, Ekhlass M., Taha, Othman, Omar, Aidil Abdul, Hamid, Abdul Jalil, Abdul Kader, Mohd Sahaid, Kalil, Gamma-Linolenic Acid Mortierella-Isabellina Malic Enzyme Echinulata Glucose Microorganisms Biosynthesis Growth Sugars Fungi Strategic feeding of ammonium and metal ions (Mg2+, Mn2+, Fe3+, Cu2+, Ca2+, Co2+, and Zn2+) for enhanced GLA-rich lipid accumulation in C. bainieri 2A1 was established. When cultivated in nitrogen-limitedmedium, the fungus produced up to 30% lipid (g/g biomass) with 12.9% (g/g lipid) GLA. However, the accumulation of lipid stopped at 48 hours of cultivation although glucose was abundant. This event occurred in parallel to the diminishing activity of malic enzyme (ME), fatty acid synthase (FAS), and ATP citrate lyase (ACL) as well as the depletion of metal ions in the medium. Reinstatement of the enzymes activities was achieved by feeding of ammonium tartrate, but no increment in the lipid content was observed. However, increment in lipid content from 32% to 50% (g/g biomass) with 13.2% GLA was achieved when simultaneous feeding of ammonium, glucose, and metal ions was carried out. This showed that the cessation of lipid accumulation was caused by diminishing activities of the enzymes as well as depletion of the metal ions in the medium. Therefore, strategic feeding of ammonium and metal ions successfully reinstated enzymes activities and enhanced GLA-rich lipid accumulation in C. bainieri 2A1. 2015-06-19T02:21:37Z 2015-06-19T02:21:37Z 2014 Article 1537-744X http://ddms.usim.edu.my/handle/123456789/8429 en_US Hindawi Publishing Corporation |
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Gamma-Linolenic Acid Mortierella-Isabellina Malic Enzyme Echinulata Glucose Microorganisms Biosynthesis Growth Sugars Fungi |
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Gamma-Linolenic Acid Mortierella-Isabellina Malic Enzyme Echinulata Glucose Microorganisms Biosynthesis Growth Sugars Fungi Shuwahida, Shuib, Wan Nazatul Naziah, Wan Nawi, Ekhlass M., Taha, Othman, Omar, Aidil Abdul, Hamid, Abdul Jalil, Abdul Kader, Mohd Sahaid, Kalil, Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 |
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Strategic feeding of ammonium and metal ions (Mg2+, Mn2+, Fe3+, Cu2+, Ca2+, Co2+, and Zn2+) for enhanced GLA-rich lipid accumulation in C. bainieri 2A1 was established. When cultivated in nitrogen-limitedmedium, the fungus produced up to 30% lipid (g/g biomass) with 12.9% (g/g lipid) GLA. However, the accumulation of lipid stopped at 48 hours of cultivation although glucose was abundant. This event occurred in parallel to the diminishing activity of malic enzyme (ME), fatty acid synthase (FAS), and ATP citrate lyase (ACL) as well as the depletion of metal ions in the medium. Reinstatement of the enzymes activities was achieved by feeding of ammonium tartrate, but no increment in the lipid content was observed. However, increment in lipid content from 32% to 50% (g/g biomass) with 13.2% GLA was achieved when simultaneous feeding of ammonium, glucose, and metal ions was carried out. This showed that the cessation of lipid accumulation was caused by diminishing activities of the enzymes as well as depletion of the metal ions in the medium. Therefore, strategic feeding of ammonium and metal ions successfully reinstated enzymes activities and enhanced GLA-rich lipid accumulation in C. bainieri 2A1. |
format |
Article |
author |
Shuwahida, Shuib, Wan Nazatul Naziah, Wan Nawi, Ekhlass M., Taha, Othman, Omar, Aidil Abdul, Hamid, Abdul Jalil, Abdul Kader, Mohd Sahaid, Kalil, |
author_facet |
Shuwahida, Shuib, Wan Nazatul Naziah, Wan Nawi, Ekhlass M., Taha, Othman, Omar, Aidil Abdul, Hamid, Abdul Jalil, Abdul Kader, Mohd Sahaid, Kalil, |
author_sort |
Shuwahida, Shuib, |
title |
Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 |
title_short |
Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 |
title_full |
Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 |
title_fullStr |
Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 |
title_full_unstemmed |
Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 |
title_sort |
strategic feeding of ammonium and metal ions for enhanced gla-rich lipid accumulation in cunninghamella bainieri 2a1 |
publisher |
Hindawi Publishing Corporation |
publishDate |
2015 |
url |
http://ddms.usim.edu.my/handle/123456789/8429 |
_version_ |
1645152416368164864 |
score |
13.18916 |