Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells
Background: Particular attention has been directed towards human amnion mesenchymal stem cells (HAMCs) due to their accessibility, availability and immunomodulatory properties. Therefore, the aim of the present study was to determine the temporal changes of sternness and angiogenic gene expressions...
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my.usim-84272017-02-23T03:38:10Z Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells Simat, Siti Fatimah, Geok Chin, Tan, Mohd Manzor, Nur Fariha, Abdul Rahman, Hayati, Kienhui, Chua, Ay Eeng, Tan, Endothelial Growth-Factor Blood-Vessel Formation Term Placenta In-Vitro Self-Renewal Bone-Marrow Differentiation Membrane Sox2 Tissue Background: Particular attention has been directed towards human amnion mesenchymal stem cells (HAMCs) due to their accessibility, availability and immunomodulatory properties. Therefore, the aim of the present study was to determine the temporal changes of sternness and angiogenic gene expressions of serial-passage HAMCs. Methods: HAMCs were isolated from human term placenta and cultured in serial passages in culture medium supplemented with 10% fetal bovine serum. Morphological analysis, growth kinetic and CFU-F assay of HAMCs were assessed. In vitro differentiation and the immunophenotype of HAMCs at P5 were also analyzed. Quantitative PCR was used to determine the sternness, angiogenic and endothelial gene expression of cultured HAMCs after serial passage. Results: Cultured HAMCs displayed intermediate epitheloid-fibroblastoid morphology at an initial culture and the fibroblastoid features became more pronounced in later passages. They showed high clonogenic activity and faster proliferation at later passages with colony forming efficiency of 0.88%. HAMCs were successfully differentiated into adipocytes, osteocytes and neuron-like cells. Most HAMCs expressed CD9, CD44, CD73, CD90 and HLA-A,B,C but negligibly expressed CD31, CD34, CD45, CD117 and HLA-DR,DP,DQ. After serial passage, sternness genes Oct-3/4, Sox-2, Nanog3, Rex-1, FGF-4 and FZD-9 expressions significantly decreased. Of the angiogenic genes PECAM-1, bFGF, eNOS, VEGFR-2, VEGF, and vWF expressions also decreased significantly except angiopoietin-1 which significantly increased. No significant differences were observed in ABCG-2, BST-1, nestin, PGF and HGF expressions after serial passage. Conclusion: These results suggested that cultured HAMCs could be an alternative source of stem cells and may have the potential for angiogenesis and hence its use in stem-cell based therapy. (C) 2012 Elsevier Inc. All rights reserved. 2015-06-19T02:14:46Z 2015-06-19T02:14:46Z 2013 Article 0026-2862 http://ddms.usim.edu.my/handle/123456789/8427 en_US Academic Press Inc Elsevier Science |
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Endothelial Growth-Factor Blood-Vessel Formation Term Placenta In-Vitro Self-Renewal Bone-Marrow Differentiation Membrane Sox2 Tissue |
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Endothelial Growth-Factor Blood-Vessel Formation Term Placenta In-Vitro Self-Renewal Bone-Marrow Differentiation Membrane Sox2 Tissue Simat, Siti Fatimah, Geok Chin, Tan, Mohd Manzor, Nur Fariha, Abdul Rahman, Hayati, Kienhui, Chua, Ay Eeng, Tan, Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
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Background: Particular attention has been directed towards human amnion mesenchymal stem cells (HAMCs) due to their accessibility, availability and immunomodulatory properties. Therefore, the aim of the present study was to determine the temporal changes of sternness and angiogenic gene expressions of serial-passage HAMCs. Methods: HAMCs were isolated from human term placenta and cultured in serial passages in culture medium supplemented with 10% fetal bovine serum. Morphological analysis, growth kinetic and CFU-F assay of HAMCs were assessed. In vitro differentiation and the immunophenotype of HAMCs at P5 were also analyzed. Quantitative PCR was used to determine the sternness, angiogenic and endothelial gene expression of cultured HAMCs after serial passage. Results: Cultured HAMCs displayed intermediate epitheloid-fibroblastoid morphology at an initial culture and the fibroblastoid features became more pronounced in later passages. They showed high clonogenic activity and faster proliferation at later passages with colony forming efficiency of 0.88%. HAMCs were successfully differentiated into adipocytes, osteocytes and neuron-like cells. Most HAMCs expressed CD9, CD44, CD73, CD90 and HLA-A,B,C but negligibly expressed CD31, CD34, CD45, CD117 and HLA-DR,DP,DQ. After serial passage, sternness genes Oct-3/4, Sox-2, Nanog3, Rex-1, FGF-4 and FZD-9 expressions significantly decreased. Of the angiogenic genes PECAM-1, bFGF, eNOS, VEGFR-2, VEGF, and vWF expressions also decreased significantly except angiopoietin-1 which significantly increased. No significant differences were observed in ABCG-2, BST-1, nestin, PGF and HGF expressions after serial passage. Conclusion: These results suggested that cultured HAMCs could be an alternative source of stem cells and may have the potential for angiogenesis and hence its use in stem-cell based therapy. (C) 2012 Elsevier Inc. All rights reserved. |
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Article |
author |
Simat, Siti Fatimah, Geok Chin, Tan, Mohd Manzor, Nur Fariha, Abdul Rahman, Hayati, Kienhui, Chua, Ay Eeng, Tan, |
author_facet |
Simat, Siti Fatimah, Geok Chin, Tan, Mohd Manzor, Nur Fariha, Abdul Rahman, Hayati, Kienhui, Chua, Ay Eeng, Tan, |
author_sort |
Simat, Siti Fatimah, |
title |
Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
title_short |
Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
title_full |
Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
title_fullStr |
Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
title_full_unstemmed |
Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
title_sort |
stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells |
publisher |
Academic Press Inc Elsevier Science |
publishDate |
2015 |
url |
http://ddms.usim.edu.my/handle/123456789/8427 |
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1645152416072466432 |
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13.18916 |