Organotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regeneration

• Main Authors: Simat, Siti Fatimah,, Geok Chin, Tan,, Ay Eeng, Tan,, Kienhui, Chua,, Tengku Ibrahim, Azmi,, Hayati, Abdul Rahman,
• Format: Article
• Language: English
• Published: Informa Healthcare 2015
• Subjects: Air-Liquid Interface; Epithelial Stem Cells; Fibrin; Human Amnion-Derived Stem Cells; Organotypic Culture; Skin Regeneration
• Online Access: http://ddms.usim.edu.my/handle/123456789/8334

Background aims. The aim of the present study was to evaluate the effects of air-liquid interface on the differentiation potential of human amnion epithelial cells (HAECs) to skin-like substitute in organotypic culture. Methods. HAECs at passage 1-2 were seeded onto a fibrin layer populated with hum...