Determination of Ammonium Ion Using a Reagentless Amperometric Biosensor Based on Immobilized Alanine Dehydrogenase

• Main Authors: Ling Ling, Tan,, Yook Heng, Lee,, Ahmad, Musa,
• Format: Article
• Language: English
• Published: Mdpi Ag 2015
• Subjects: Biosensor; Ammonium Ion; Reduced Nicotinamide Adenine Dinucleotide; Poly(2-Hydroxyethyl Methacrylate; Alanine Dehydrogenase

The use of the enzyme alanine dehydrogenase (AlaDH) for the determination of ammonium ion (NH(4)(+))usually requires the addition of pyruvate substrate and reduced nicotinamide adenine dinucleotide (NADH) simultaneously to effect the reaction. This addition of reagents is inconvenient when an enzyme...