Halal Microcarrier Preparation for Vaccine Manufacturing
Microcarriers are tiny beads or particles (matrix) with a surface chemistry that facilitates attachment and growth of anchorage-dependent cells in cell culture processes. Tuning the interactions of cells to the engineered matrix is a major challenge in cell and tissue engineering. The aim of this...
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my.usim-61232015-07-03T01:02:35Z Halal Microcarrier Preparation for Vaccine Manufacturing A.N. Yusilawati M. Maizirwan I. Sopyan M.S. Hamzah K.H. Ng C.S. Wong Polymer--Polystyrene--UV/ozone--Modification Microcarriers are tiny beads or particles (matrix) with a surface chemistry that facilitates attachment and growth of anchorage-dependent cells in cell culture processes. Tuning the interactions of cells to the engineered matrix is a major challenge in cell and tissue engineering. The aim of this study was to develop polymeric microcarrier to obtain higher cell density in bioreactor culture for vaccine manufacturing from animal cell thus ensuring the whole process is halal. This cell-carrier was developed from micro-size polystyrene beads (100-150µm) which have been surface modified by UV/ozone treatment and covalent immobilization of by gelatin (bovine and fish) on the material surface. Cell loading test in Vero cell culture was performed to evaluate their performance as carrier system. It was found that the system used was effective to develop gelatin coated polystyrene beads having good microscopic and suspension ability, good surface for cell attachment and proliferation, easy sampling and easy cell recovery with highest maximum cell number obtained with no toxicity to the cells. The novelty of this product includes simple and improved technique to prepare halal microcarrier with cheaper production cost. In addition, less concentration of microcarrier (3g/L) can be used for cell culture compared to commercial microcarrier (4g/L - 20g/L). Thus, this matrix can be used to substitute commercial microcarriers which were developed from non-halal resources (porcine gelatin) such as Cytodex 3, FACT III, Collagen from Solo Hill, CGEN 102-L and many more. Therefore, the product is applicable for large scale production and ready for commercialization. 2013-01-04T07:31:10Z 2013-01-04T07:31:10Z 2013-01-04 Conference Paper http://ddms.usim.edu.my/handle/123456789/6123 en |
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Polymer--Polystyrene--UV/ozone--Modification A.N. Yusilawati M. Maizirwan I. Sopyan M.S. Hamzah K.H. Ng C.S. Wong Halal Microcarrier Preparation for Vaccine Manufacturing |
description |
Microcarriers are tiny beads or particles (matrix) with a surface chemistry that facilitates
attachment and growth of anchorage-dependent cells in cell culture processes. Tuning the
interactions of cells to the engineered matrix is a major challenge in cell and tissue
engineering. The aim of this study was to develop polymeric microcarrier to obtain higher
cell density in bioreactor culture for vaccine manufacturing from animal cell thus ensuring
the whole process is halal. This cell-carrier was developed from micro-size polystyrene
beads (100-150µm) which have been surface modified by UV/ozone treatment and
covalent immobilization of by gelatin (bovine and fish) on the material surface. Cell loading
test in Vero cell culture was performed to evaluate their performance as carrier system. It
was found that the system used was effective to develop gelatin coated polystyrene beads
having good microscopic and suspension ability, good surface for cell attachment and
proliferation, easy sampling and easy cell recovery with highest maximum cell number
obtained with no toxicity to the cells. The novelty of this product includes simple and
improved technique to prepare halal microcarrier with cheaper production cost. In addition,
less concentration of microcarrier (3g/L) can be used for cell culture compared to
commercial microcarrier (4g/L - 20g/L). Thus, this matrix can be used to substitute
commercial microcarriers which were developed from non-halal resources (porcine
gelatin) such as Cytodex 3, FACT III, Collagen from Solo Hill, CGEN 102-L and many
more. Therefore, the product is applicable for large scale production and ready for
commercialization. |
format |
Conference Paper |
author |
A.N. Yusilawati M. Maizirwan I. Sopyan M.S. Hamzah K.H. Ng C.S. Wong |
author_facet |
A.N. Yusilawati M. Maizirwan I. Sopyan M.S. Hamzah K.H. Ng C.S. Wong |
author_sort |
A.N. Yusilawati |
title |
Halal Microcarrier Preparation for Vaccine Manufacturing |
title_short |
Halal Microcarrier Preparation for Vaccine Manufacturing |
title_full |
Halal Microcarrier Preparation for Vaccine Manufacturing |
title_fullStr |
Halal Microcarrier Preparation for Vaccine Manufacturing |
title_full_unstemmed |
Halal Microcarrier Preparation for Vaccine Manufacturing |
title_sort |
halal microcarrier preparation for vaccine manufacturing |
publishDate |
2013 |
url |
http://ddms.usim.edu.my/handle/123456789/6123 |
_version_ |
1645151812731273216 |
score |
13.214268 |