Development of a T7 RNA polymerase expressing cell line using lentivirus vectors for the recovery of recombinant Newcastle disease virus

• Main Authors: Yeong, Ming Yue, Cheow, Pheik Sheen, Abdullah, Syahril, Song, Adelene Ai Lian, Lei, Janet Rossmann, Tan, Tiong Kit, Yusoff, Khatijah, Chia, Suet Lin
• Format: Article
• Language: English
• Published: Elsevier 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/96691/1/ABSTRACT.pdf; http://psasir.upm.edu.my/id/eprint/96691/; https://www.sciencedirect.com/science/article/pii/S0166093421000380

The development of a T7 RNA polymerase (T7 RNAP) expressing cell line i.e. BSR T7/5 cells marks an improvement of reverse genetics for the recovery of recombinant Newcastle disease virus (rNDV). BSR T7/5 is developed by transient transfection of plasmid encoding T7 RNAP gene for rNDV rescue. However...