The involvement of L-arginine-nitric ox-ide-cGMP-ATP-sensitive K+ channel pathway in antinocicep-tion of BBHC, a novel diarylpentanoid analogue, in mice model

• Main Authors: Hui, Ming Ong, Ahmad Azmi, Ahmad Farhan, Sze, Wei Leong, Abas, Faridah, Perimal, Enoch Kumar, Omar Farouk, Ahmad Akira, Israf Ali, Daud Ahmad, Sulaiman, Mohd Roslan
• Format: Article
• Language: English
• Published: MDPI AG 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/95511/1/The%20involvement%20of%20L-arginine-nitric%20ox-ide-cGMP-ATP-sensitive%20K%2B%20channel%20pathway%20in%20antinocicep-tion%20of%20BBHC%2C%20a%20novel%20diarylpentanoid%20analogue%2C%20in%20mice%20model.pdf; http://psasir.upm.edu.my/id/eprint/95511/; https://www.mdpi.com/1420-3049/26/24/7431

The present study focuses on the possible involvement of l-arginine-nitric oxide-cGMP-ATP-sensitive K+ channel pathway in the antinociceptive activity of a novel diarylpentanoid analogue, 2-benzoyl-6-(3-bromo-4-hydroxybenzylidene)cyclohexen-1-ol (BBHC) via a chemical nociceptive model in mice. The antinociceptive action of BBHC (1 mg/kg, i.p.) was attenuated by the intraperitoneal pre-treatment of l-arginine (a nitric oxide synthase precursor) and glibenclamide (an ATP-sensitive K+ channel blocker) in acetic acid-induced abdominal constriction tests. Interestingly, BBHC’s antinociception was significantly enhanced by the i.p. pre-treatment of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a selective inhibitor of soluble guanylyl cyclase (p < 0.05). Altogether, these findings suggest that the systemic administration of BBHC is able to establish a significant antinociceptive effect in a mice model of chemically induced pain. BBHC’s antinociception is shown to be mediated by the involvement of l-arginine-nitric oxide-cGMP-ATP-sensitive K+ channel pathway, without any potential sedative or muscle relaxant concerns.