Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract
The use of metabolomics as a comprehensive tool in the analysis of metabolic profiles in disease progression and therapeutic intervention is rapidly advancing. Yet, a single analytical platform could not be applied to cover the entire spectrum of a biological sample’s metabolome. In the present p...
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my.upm.eprints.942952023-05-03T09:04:15Z http://psasir.upm.edu.my/id/eprint/94295/ Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract Ahmad Azam, Amalina Ismail, Intan Safinar Shaikh, Mohd Farooq Abas, Faridah Shaari, Khozirah The use of metabolomics as a comprehensive tool in the analysis of metabolic profiles in disease progression and therapeutic intervention is rapidly advancing. Yet, a single analytical platform could not be applied to cover the entire spectrum of a biological sample’s metabolome. In the present paper, multi-platform metabolomics approaches were explored to determine the diverse rat sera metabolites extracted from intracerebroventricular lipopolysaccharides (LPS)-induced neuroinflammed rats treated with oral therapeutic interventions of positive drug (dextromethorphan, 5 mg/kg BW); with Clinacanthus nutans (CN) aqueous extract (CNE, 500 mg/kg BW); and with phosphate buffer saline (PBS) as the control group for 14 days. Analyzed by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) techniques, this study depicted the potential of metabolites associated with neuroinflammation and verified by MetDisease. The key observations in the perturbed metabolic pathways that showed ameliorative effects were linked to the class of amino acid and peptide metabolism involving valine, leucine, and isoleucine biosynthesis; phenylalanine, tyrosine, and tryptophan biosynthesis; and phenylalanine metabolism. Lipid metabolism of arachidonic acid metabolism, glycerophospholipid metabolism, terpenoid backbone biosynthesis, and glycosphingolipid metabolism were also affected. Current findings suggested that the putative biomarkers, especially lysophosphatidic acid (LPA) and 5- diphosphomevalonic acid from glycerophospholipid and squalene/terpenoid and cholesterol biosynthesis, respectively, showed the ameliorative effects of the drug and CN treatments by controlling cell differentiation and proliferation. Our study proved that the complex and dynamic sera profiling affected during the CN treatment was greatly influenced by the analytical platform selection as integration between the two data yielded a more holistic summary of the metabolite pattern changes. Hence, an evidence-based herb, such as CN, can be used for novel diagnostic tools in the quest for ethnopharmacological studies. Frontiers Media 2021-06-09 Article PeerReviewed Ahmad Azam, Amalina and Ismail, Intan Safinar and Shaikh, Mohd Farooq and Abas, Faridah and Shaari, Khozirah (2021) Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract. Frontiers in Pharmacology, 12. art. no. 629561. pp. 1-15. ISSN 1663-9812 https://www.frontiersin.org/articles/10.3389/fphar.2021.629561/full 10.3389/fphar.2021.629561 |
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The use of metabolomics as a comprehensive tool in the analysis of metabolic profiles in
disease progression and therapeutic intervention is rapidly advancing. Yet, a single
analytical platform could not be applied to cover the entire spectrum of a biological
sample’s metabolome. In the present paper, multi-platform metabolomics approaches
were explored to determine the diverse rat sera metabolites extracted from
intracerebroventricular lipopolysaccharides (LPS)-induced neuroinflammed rats treated
with oral therapeutic interventions of positive drug (dextromethorphan, 5 mg/kg BW); with
Clinacanthus nutans (CN) aqueous extract (CNE, 500 mg/kg BW); and with phosphate
buffer saline (PBS) as the control group for 14 days. Analyzed by nuclear magnetic
resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) techniques,
this study depicted the potential of metabolites associated with neuroinflammation and
verified by MetDisease. The key observations in the perturbed metabolic pathways that
showed ameliorative effects were linked to the class of amino acid and peptide metabolism
involving valine, leucine, and isoleucine biosynthesis; phenylalanine, tyrosine, and
tryptophan biosynthesis; and phenylalanine metabolism. Lipid metabolism of
arachidonic acid metabolism, glycerophospholipid metabolism, terpenoid backbone
biosynthesis, and glycosphingolipid metabolism were also affected. Current findings
suggested that the putative biomarkers, especially lysophosphatidic acid (LPA) and 5-
diphosphomevalonic acid from glycerophospholipid and squalene/terpenoid and
cholesterol biosynthesis, respectively, showed the ameliorative effects of the drug and
CN treatments by controlling cell differentiation and proliferation. Our study proved that the
complex and dynamic sera profiling affected during the CN treatment was greatly
influenced by the analytical platform selection as integration between the two data
yielded a more holistic summary of the metabolite pattern changes. Hence, an
evidence-based herb, such as CN, can be used for novel diagnostic tools in the quest
for ethnopharmacological studies. |
format |
Article |
author |
Ahmad Azam, Amalina Ismail, Intan Safinar Shaikh, Mohd Farooq Abas, Faridah Shaari, Khozirah |
spellingShingle |
Ahmad Azam, Amalina Ismail, Intan Safinar Shaikh, Mohd Farooq Abas, Faridah Shaari, Khozirah Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract |
author_facet |
Ahmad Azam, Amalina Ismail, Intan Safinar Shaikh, Mohd Farooq Abas, Faridah Shaari, Khozirah |
author_sort |
Ahmad Azam, Amalina |
title |
Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract |
title_short |
Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract |
title_full |
Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract |
title_fullStr |
Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract |
title_full_unstemmed |
Multi-platform metabolomics analyses revealed the complexity of serum metabolites in LPS-induced neuroinflammed rats treated with Clinacanthus nutans aqueous extract |
title_sort |
multi-platform metabolomics analyses revealed the complexity of serum metabolites in lps-induced neuroinflammed rats treated with clinacanthus nutans aqueous extract |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
http://psasir.upm.edu.my/id/eprint/94295/ https://www.frontiersin.org/articles/10.3389/fphar.2021.629561/full |
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1765298657123368960 |
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13.19449 |