Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
In this study, a mixture of Tor tambra and T. tambroides with unknown genetic background were collected from 11 localities in Malaysia for broodstock development and sperm cryo-banking. This study aims to assess the microsatellite (simple sequence repeat, SSR) variation, genetic diversity, genetic d...
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my.upm.eprints.941872023-05-09T03:12:13Z http://psasir.upm.edu.my/id/eprint/94187/ Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization Chew, Poh Chiang Christianus, Annie M. Zudaidy, Jaapar Md Yasin, Ina-Salwany Chong, Chou Min Tan, Soon Guan In this study, a mixture of Tor tambra and T. tambroides with unknown genetic background were collected from 11 localities in Malaysia for broodstock development and sperm cryo-banking. This study aims to assess the microsatellite (simple sequence repeat, SSR) variation, genetic diversity, genetic differentiation, level of gene flow, population structure, genetic relatedness and their demographic aspects among these Tor populations, in addition to establishing their SSR profile by employing 22 SSR markers via fragment analysis. Total genomic DNA was extracted from 181 samples (91 cryopreserved milt samples and 90 scale samples of live broodfish). Results showed the Tor spp. collection retained their genetic variation but exhibited excessive homozygosity among individuals within population. Moderate genetic differentiation was shown among the populations, with highly significant (p < 0.001) fixation indices (FST, FIS and FIT). A low gene flow over all loci (Nm 1.548) indicates little genetic variation transfer between populations. The genetic structures of all the populations were successfully resolved into four main clusters by an unweighted pair group method with arithmetic mean (UPGMA) dendrogram generated based on Nei’s genetic distances. The population structures based on principal coordinates analysis (PCoA) and the Bayesian model also suggested four distinct clusters following geographical regions and eight closely related populations. This study provided a useful baseline reference for better genetic management and utilization of the Tor spp. stocks in their breeding and conservation programmes. MDPI 2021-09-08 Article PeerReviewed Chew, Poh Chiang and Christianus, Annie and M. Zudaidy, Jaapar and Md Yasin, Ina-Salwany and Chong, Chou Min and Tan, Soon Guan (2021) Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization. Animals, 11 (9). art. no. 2633. pp. 1-28. ISSN 2076-2615 https://www.mdpi.com/2076-2615/11/9/2633 10.3390/ani11092633 |
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In this study, a mixture of Tor tambra and T. tambroides with unknown genetic background were collected from 11 localities in Malaysia for broodstock development and sperm cryo-banking. This study aims to assess the microsatellite (simple sequence repeat, SSR) variation, genetic diversity, genetic differentiation, level of gene flow, population structure, genetic relatedness and their demographic aspects among these Tor populations, in addition to establishing their SSR profile by employing 22 SSR markers via fragment analysis. Total genomic DNA was extracted from 181 samples (91 cryopreserved milt samples and 90 scale samples of live broodfish). Results showed the Tor spp. collection retained their genetic variation but exhibited excessive homozygosity among individuals within population. Moderate genetic differentiation was shown among the populations, with highly significant (p < 0.001) fixation indices (FST, FIS and FIT). A low gene flow over all loci (Nm 1.548) indicates little genetic variation transfer between populations. The genetic structures of all the populations were successfully resolved into four main clusters by an unweighted pair group method with arithmetic mean (UPGMA) dendrogram generated based on Nei’s genetic distances. The population structures based on principal coordinates analysis (PCoA) and the Bayesian model also suggested four distinct clusters following geographical regions and eight closely related populations. This study provided a useful baseline reference for better genetic management and utilization of the Tor spp. stocks in their breeding and conservation programmes. |
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Chew, Poh Chiang Christianus, Annie M. Zudaidy, Jaapar Md Yasin, Ina-Salwany Chong, Chou Min Tan, Soon Guan |
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Chew, Poh Chiang Christianus, Annie M. Zudaidy, Jaapar Md Yasin, Ina-Salwany Chong, Chou Min Tan, Soon Guan Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization |
author_facet |
Chew, Poh Chiang Christianus, Annie M. Zudaidy, Jaapar Md Yasin, Ina-Salwany Chong, Chou Min Tan, Soon Guan |
author_sort |
Chew, Poh Chiang |
title |
Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization |
title_short |
Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization |
title_full |
Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization |
title_fullStr |
Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization |
title_full_unstemmed |
Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization |
title_sort |
microsatellite characterization of malaysian mahseer (tor spp.) for improvement of broodstock management and utilization |
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MDPI |
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2021 |
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http://psasir.upm.edu.my/id/eprint/94187/ https://www.mdpi.com/2076-2615/11/9/2633 |
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