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Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP

Orthosiphon stamineus which belongs to the Lamiaceae family is an important herb. A study was conducted to determine the best sterilization procedure for in vitro culture of Orthosiphon stamineus using shoot tip explant. Different concentrations of Clorox at different immersion time were used to opt...

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Main Author: Borhan, Nur Hayyu
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/91442/1/FP%202016%2074%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91442/
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spelling my.upm.eprints.914422021-11-18T01:08:24Z http://psasir.upm.edu.my/id/eprint/91442/ Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP Borhan, Nur Hayyu Orthosiphon stamineus which belongs to the Lamiaceae family is an important herb. A study was conducted to determine the best sterilization procedure for in vitro culture of Orthosiphon stamineus using shoot tip explant. Different concentrations of Clorox at different immersion time were used to optimize the sterilization procedure. Immersion of shoot tip explant in 25% concentration of Clorox for 15 minutes reduced microbial contamination up to 10% but increase explant survivability. Another study was conducted by culturing the shoot tip of Orthosiphon stamineus in half strength MS media containing different BAP concentration. The media containing 1 mg/L of BAP gave the highest shoot proliferation in shoot tip culture of Orthosiphon stamineus with 12.3 shoot produced per explant. 2016 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91442/1/FP%202016%2074%20IR.pdf Borhan, Nur Hayyu (2016) Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Orthosiphon stamineus which belongs to the Lamiaceae family is an important herb. A study was conducted to determine the best sterilization procedure for in vitro culture of Orthosiphon stamineus using shoot tip explant. Different concentrations of Clorox at different immersion time were used to optimize the sterilization procedure. Immersion of shoot tip explant in 25% concentration of Clorox for 15 minutes reduced microbial contamination up to 10% but increase explant survivability. Another study was conducted by culturing the shoot tip of Orthosiphon stamineus in half strength MS media containing different BAP concentration. The media containing 1 mg/L of BAP gave the highest shoot proliferation in shoot tip culture of Orthosiphon stamineus with 12.3 shoot produced per explant.
format Project Paper Report
author Borhan, Nur Hayyu
spellingShingle Borhan, Nur Hayyu
Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP
author_facet Borhan, Nur Hayyu
author_sort Borhan, Nur Hayyu
title Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP
title_short Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP
title_full Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP
title_fullStr Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP
title_full_unstemmed Surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (Orthosiphon stamineus) using different concentration of Clorox and BAP
title_sort surface sterilization and shoot proliferation from shoot tip explant of cat's whiskers (orthosiphon stamineus) using different concentration of clorox and bap
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/91442/1/FP%202016%2074%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91442/
_version_ 1717095445894791168
score 13.159267

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