Plasmid characterization of Enterobacter sp. From human breast milk in Malaysia
Enterobacter sp. species are nosocomial pathogens that could cause variety of infections, such as pneumonia, urinary tract infections or surgical wound infections. This ability in infecting human can cause by present of antibiotic-resistant plasmids which resistant toward antibiotics and this bec...
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| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/91035/1/FBSB%202015%20145%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/91035/ |
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| Summary: | Enterobacter sp. species are nosocomial pathogens that could cause variety of
infections, such as pneumonia, urinary tract infections or surgical wound infections.
This ability in infecting human can cause by present of antibiotic-resistant plasmids
which resistant toward antibiotics and this become a challenging clinical problem.
The aim of this study is to isolate and characterize the plasmid extracted from
Enterobacter cloacae from breast milk in Malaysian women. Extraction method of
modified alkaline lysis and using Promega PureYield™ Plasmid Miniprep System
were used to obtain high concentration of plasmids. The product size was expected to
be ~11kbp and in this experiment, highly purified and concentrated plasmids were
difficult to obtain thus, several modifications in plasmid extraction a required. PCR
reaction was run using M13 universal primers and send for sequencing for the
fragments which being amplify. This project was also being carried out to design a
more versatile plasmid DNA which might aid in cloning system. |
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