Effects of plant growth – promoting rhizobacteria and nitrogen on growth of pegaga (Centella asiatica (L.)) in a hydroponic system
Centella asiatica (L.), pegaga, is a small creeping herb. It is one of the top 5 Malaysian herbs categorized for their medicinal quality to enhance the national income. C. asiatica is known to aid in the treatments of wounds, ulcers, skin diseases, tuberculosis and various mental...
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Format: | Project Paper Report |
Language: | English |
Published: |
2015
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Online Access: | http://psasir.upm.edu.my/id/eprint/90110/1/FP%202015%20142%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/90110/ |
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Summary: | Centella asiatica (L.), pegaga, is a small creeping herb. It is one of the top 5 Malaysian herbs
categorized for their medicinal quality to enhance the national income. C. asiatica is known to
aid in the treatments of wounds, ulcers, skin diseases, tuberculosis and various mental
disorders. Plant Growth-Promoting Rhizobacteria (PGPR) are defined as root colonizing bacteria
which increase plant nutrient uptake and promote plant growth and development. This experiment was
carried out to study the effects of different PGPR and nitrogen levels on growth of C. asiatica
under hydroponic system. The objective of this experiment was to determine the best PGPR
strain and the optimum level of nitrogen for increased vegetative growth of C. asiatica
under hydroponic system. The three selected PGPR strains (UPMB10, Rhizospheric and
Endophytic pegaga isolates)were inoculated toC. asiatica with three different levels of nitrogen
(0%, 33% and 100%)in test tubes and left for 21 days in a plant growth chamber with
twelve treatments and three replications using a Completely Randomized Design (CRD).The
parameters were incremental height and leaf greenness (SPAD values). The data were analyzed
using ANOVA and the means were compared by using Tukey test. At the end of the experiment, it was
observed that there was no significant difference between all treatments for incremental height
(growth rate) at week 1 and week 3 and leaf greenness (SPAD values). However, a significant
difference was observed in incremental height (growth rate) between N levels at week 2.
The best PGPR strain that have a good relationship with the optimum usage of nitrogen for
C.asiatica were U strain (UPMB- 10) and E strain (endophytic) with 0 % nitrogen, which had higher
incremental heights
and greenness of leaves compared to R strain. |
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