Lack of methylation on transgene leads to high level and persistent transgene expression in induced pluripotent stem cells

• Main Authors: Alhaji, Suleiman Yusuf, Nordin, Norshariza, Ngai, Siew Ching, Al Abbar, Akram, Lai, Mei I., Abdullah, Syahril
• Format: Article
• Language: English
• Published: Elsevier 2020
• Online Access: http://psasir.upm.edu.my/id/eprint/89539/1/LENTI.pdf; http://psasir.upm.edu.my/id/eprint/89539/; https://www.sciencedirect.com/science/article/pii/S0378111920306272

Short-lived therapeutic gene expression in mammalian cells by DNA methylation is one of the major challenges in gene therapy. In this study, we assessed the implication of DNA methylation on the duration of GFP expression in mouse embryonic stem (ES) and mouse induced pluripotent stem (iPS) cells. T...