Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey
Kelulut honey (KH) is a natural product-derived food produced by stingless bees of Trigona or Meliponine species. Several studies have shown that the geographical origin of honey significantly affects its pharmacological properties. Thus, this study aims to characterise the pharmacological propertie...
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2020
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my.upm.eprints.891492021-09-01T21:57:27Z http://psasir.upm.edu.my/id/eprint/89149/ Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey Badrulhisham, Nurul Sharina Rasyidah Ab Hamid, Siti Nur Parvin Ismail, Muhammad Aiman Haiqal Yong, Yoke Keong Muhamad Zakuan, Noraina Harith, Hanis Hazeera Saidi, Hasni Idayu Nurdin, Armania Kelulut honey (KH) is a natural product-derived food produced by stingless bees of Trigona or Meliponine species. Several studies have shown that the geographical origin of honey significantly affects its pharmacological properties. Thus, this study aims to characterise the pharmacological properties of KH harvested from different geographical locations. The total phenolic content (TPC), antioxidant levels, cytotoxic, and anti-inflammatory activities of pure KH harvested from three different locations (Sarawak, Pahang and Selangor) were compared. Among the samples, KH harvested from Selangor exhibited the highest TPC, antioxidant levels, and cytotoxic activity against MDA-MB-231 and MCF-7 cells, followed by KH harvested from Sarawak and Pahang. The IC50 of MCF-7 cells treated with KH harvested from Selangor was at least 2-fold lower than the IC50 of MDA-MB-231 cells, suggesting that KH is more cytotoxic to oestrogen receptor (ER)- and progesterone receptor (PR)-positive (MCF-7) compared to triple-negative (MDA-MB-231) breast cancer cells. Two non-cytotoxic concentrations (1% and 0.5%; v/v) were selected for the anti-inflammatory assay using lipopolysaccharide (LPS)-induced RAW 264.7 cells. KH harvested from Selangor and Pahang (at a concentration of 1%; v/v) significantly inhibited nitric oxide (NO) production in LPS-induced RAW 264.7 cells compared to control cells. These findings provide evidence that the geographical origin of KH may indeed influence its pharmacological properties. Our data suggest that KH harvested from Selangor has a better quality compared to KH harvested from Pahang and Sarawak based on its high TPC, antioxidant levels and anti-inflammatory activity. Furthermore, it exhibits in vitro anticancer potential in breast cancer cell lines. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89149/1/HONEY.pdf Badrulhisham, Nurul Sharina Rasyidah and Ab Hamid, Siti Nur Parvin and Ismail, Muhammad Aiman Haiqal and Yong, Yoke Keong and Muhamad Zakuan, Noraina and Harith, Hanis Hazeera and Saidi, Hasni Idayu and Nurdin, Armania (2020) Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey. Journal of Asia-Pacific Entomology, 23 (4). 950 - 956. ISSN 1226-8615 https://www.sciencedirect.com/science/article/pii/S1226861520301771 10.1016/j.aspen.2020.07.015 |
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Kelulut honey (KH) is a natural product-derived food produced by stingless bees of Trigona or Meliponine species. Several studies have shown that the geographical origin of honey significantly affects its pharmacological properties. Thus, this study aims to characterise the pharmacological properties of KH harvested from different geographical locations. The total phenolic content (TPC), antioxidant levels, cytotoxic, and anti-inflammatory activities of pure KH harvested from three different locations (Sarawak, Pahang and Selangor) were compared. Among the samples, KH harvested from Selangor exhibited the highest TPC, antioxidant levels, and cytotoxic activity against MDA-MB-231 and MCF-7 cells, followed by KH harvested from Sarawak and Pahang. The IC50 of MCF-7 cells treated with KH harvested from Selangor was at least 2-fold lower than the IC50 of MDA-MB-231 cells, suggesting that KH is more cytotoxic to oestrogen receptor (ER)- and progesterone receptor (PR)-positive (MCF-7) compared to triple-negative (MDA-MB-231) breast cancer cells. Two non-cytotoxic concentrations (1% and 0.5%; v/v) were selected for the anti-inflammatory assay using lipopolysaccharide (LPS)-induced RAW 264.7 cells. KH harvested from Selangor and Pahang (at a concentration of 1%; v/v) significantly inhibited nitric oxide (NO) production in LPS-induced RAW 264.7 cells compared to control cells. These findings provide evidence that the geographical origin of KH may indeed influence its pharmacological properties. Our data suggest that KH harvested from Selangor has a better quality compared to KH harvested from Pahang and Sarawak based on its high TPC, antioxidant levels and anti-inflammatory activity. Furthermore, it exhibits in vitro anticancer potential in breast cancer cell lines. |
format |
Article |
author |
Badrulhisham, Nurul Sharina Rasyidah Ab Hamid, Siti Nur Parvin Ismail, Muhammad Aiman Haiqal Yong, Yoke Keong Muhamad Zakuan, Noraina Harith, Hanis Hazeera Saidi, Hasni Idayu Nurdin, Armania |
spellingShingle |
Badrulhisham, Nurul Sharina Rasyidah Ab Hamid, Siti Nur Parvin Ismail, Muhammad Aiman Haiqal Yong, Yoke Keong Muhamad Zakuan, Noraina Harith, Hanis Hazeera Saidi, Hasni Idayu Nurdin, Armania Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
author_facet |
Badrulhisham, Nurul Sharina Rasyidah Ab Hamid, Siti Nur Parvin Ismail, Muhammad Aiman Haiqal Yong, Yoke Keong Muhamad Zakuan, Noraina Harith, Hanis Hazeera Saidi, Hasni Idayu Nurdin, Armania |
author_sort |
Badrulhisham, Nurul Sharina Rasyidah |
title |
Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
title_short |
Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
title_full |
Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
title_fullStr |
Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
title_full_unstemmed |
Harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
title_sort |
harvested locations influence the total phenolic content, antioxidant levels, cytotoxic, and anti-inflammatory activities of stingless bee honey |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/89149/1/HONEY.pdf http://psasir.upm.edu.my/id/eprint/89149/ https://www.sciencedirect.com/science/article/pii/S1226861520301771 |
_version_ |
1710677167688384512 |
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13.18916 |