Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin
Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a. Initially, the endolysin was cloned using BamHI/XhoI, resulti...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88588/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/88588/ https://www.future-science.com/doi/10.2144/btn-2020-0034 |
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