Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia

Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR...

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Main Authors: Darshanie, Tennakon Mudiyanselage Nadika, Ahmad, Khairulmazmi, Kong, Lih Ling, Vadamalai, Ganesan
Format: Article
Language:English
Published: Global Society of Scientific Research and Researchers 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88381/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/88381/
https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/11264
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spelling my.upm.eprints.883812021-12-28T08:56:07Z http://psasir.upm.edu.my/id/eprint/88381/ Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia Darshanie, Tennakon Mudiyanselage Nadika Ahmad, Khairulmazmi Kong, Lih Ling Vadamalai, Ganesan Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR) using phytoplasma universal primers based on 16SrRNA and SecA gene sequences. Sequence analysis of 1.2 kb and 480 bp amplicons of the 16SrRNA and SecA gene respectively confirmed the presence of phytoplasma DNA associated with Candidatus phytoplasma asteris (Group16SrI) in the symptomatic bitter gourd samples. Phylogenetic analysis of the 16SrDNA and SecA sequences placed the bitter gourd phytoplasma in the 16SrI phytoplasma group. This is the first report of phytoplasma infection in bitter gourd in Malaysia. Global Society of Scientific Research and Researchers 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88381/1/ABSTRACT.pdf Darshanie, Tennakon Mudiyanselage Nadika and Ahmad, Khairulmazmi and Kong, Lih Ling and Vadamalai, Ganesan (2020) Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia. International Journal of Sciences: Basic and Applied Research, 52 (1). 154 - 163. ISSN 2307-4531 https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/11264
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR) using phytoplasma universal primers based on 16SrRNA and SecA gene sequences. Sequence analysis of 1.2 kb and 480 bp amplicons of the 16SrRNA and SecA gene respectively confirmed the presence of phytoplasma DNA associated with Candidatus phytoplasma asteris (Group16SrI) in the symptomatic bitter gourd samples. Phylogenetic analysis of the 16SrDNA and SecA sequences placed the bitter gourd phytoplasma in the 16SrI phytoplasma group. This is the first report of phytoplasma infection in bitter gourd in Malaysia.
format Article
author Darshanie, Tennakon Mudiyanselage Nadika
Ahmad, Khairulmazmi
Kong, Lih Ling
Vadamalai, Ganesan
spellingShingle Darshanie, Tennakon Mudiyanselage Nadika
Ahmad, Khairulmazmi
Kong, Lih Ling
Vadamalai, Ganesan
Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
author_facet Darshanie, Tennakon Mudiyanselage Nadika
Ahmad, Khairulmazmi
Kong, Lih Ling
Vadamalai, Ganesan
author_sort Darshanie, Tennakon Mudiyanselage Nadika
title Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
title_short Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
title_full Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
title_fullStr Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
title_full_unstemmed Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
title_sort detection of candidatus phytoplasma asteris’ (16sri) associated with bitter gourd leaf and floral malformations in malaysia
publisher Global Society of Scientific Research and Researchers
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88381/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/88381/
https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/11264
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