Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Global Society of Scientific Research and Researchers
2020
|
Online Access: | http://psasir.upm.edu.my/id/eprint/88381/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/88381/ https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/11264 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.upm.eprints.88381 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.883812021-12-28T08:56:07Z http://psasir.upm.edu.my/id/eprint/88381/ Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia Darshanie, Tennakon Mudiyanselage Nadika Ahmad, Khairulmazmi Kong, Lih Ling Vadamalai, Ganesan Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR) using phytoplasma universal primers based on 16SrRNA and SecA gene sequences. Sequence analysis of 1.2 kb and 480 bp amplicons of the 16SrRNA and SecA gene respectively confirmed the presence of phytoplasma DNA associated with Candidatus phytoplasma asteris (Group16SrI) in the symptomatic bitter gourd samples. Phylogenetic analysis of the 16SrDNA and SecA sequences placed the bitter gourd phytoplasma in the 16SrI phytoplasma group. This is the first report of phytoplasma infection in bitter gourd in Malaysia. Global Society of Scientific Research and Researchers 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88381/1/ABSTRACT.pdf Darshanie, Tennakon Mudiyanselage Nadika and Ahmad, Khairulmazmi and Kong, Lih Ling and Vadamalai, Ganesan (2020) Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia. International Journal of Sciences: Basic and Applied Research, 52 (1). 154 - 163. ISSN 2307-4531 https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/11264 |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR) using phytoplasma universal primers based on 16SrRNA and SecA gene sequences. Sequence analysis of 1.2 kb and 480 bp amplicons of the 16SrRNA and SecA gene respectively confirmed the presence of phytoplasma DNA associated with Candidatus phytoplasma asteris (Group16SrI) in the symptomatic bitter gourd samples. Phylogenetic analysis of the 16SrDNA and SecA sequences placed the bitter gourd phytoplasma in the 16SrI phytoplasma group. This is the first report of phytoplasma infection in bitter gourd in Malaysia. |
format |
Article |
author |
Darshanie, Tennakon Mudiyanselage Nadika Ahmad, Khairulmazmi Kong, Lih Ling Vadamalai, Ganesan |
spellingShingle |
Darshanie, Tennakon Mudiyanselage Nadika Ahmad, Khairulmazmi Kong, Lih Ling Vadamalai, Ganesan Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia |
author_facet |
Darshanie, Tennakon Mudiyanselage Nadika Ahmad, Khairulmazmi Kong, Lih Ling Vadamalai, Ganesan |
author_sort |
Darshanie, Tennakon Mudiyanselage Nadika |
title |
Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia |
title_short |
Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia |
title_full |
Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia |
title_fullStr |
Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia |
title_full_unstemmed |
Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia |
title_sort |
detection of candidatus phytoplasma asteris’ (16sri) associated with bitter gourd leaf and floral malformations in malaysia |
publisher |
Global Society of Scientific Research and Researchers |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/88381/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/88381/ https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/11264 |
_version_ |
1724075504059809792 |
score |
13.214268 |