Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm

Basal stem rot (BSR) disease caused by Ganoderma boninense basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environm...

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Main Authors: Goh, Yit Kheng, Marzuki, Nurul Fadhilah, Tuan Pa, Tuan Nur Fatihah, Goh, Teik Khiang, Kee, Zeng Seng, Goh, You Keng, Yusof, Mohd Termizi, Vujanovic, Vladimir, Goh, Kah Joo
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88027/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/88027/
https://www.mdpi.com/2076-2607/8/8/1138
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spelling my.upm.eprints.880272022-05-24T04:49:44Z http://psasir.upm.edu.my/id/eprint/88027/ Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm Goh, Yit Kheng Marzuki, Nurul Fadhilah Tuan Pa, Tuan Nur Fatihah Goh, Teik Khiang Kee, Zeng Seng Goh, You Keng Yusof, Mohd Termizi Vujanovic, Vladimir Goh, Kah Joo Basal stem rot (BSR) disease caused by Ganoderma boninense basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environmentally friendly biocontrol approaches in minimizing BSR disease. This study investigated the performance of two potential biocontrol agents (BCAs), AAT0115 and AAB0114 strains recovered from oil palm on suppression of BSR in planta, and also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated the two ascomycetous Talaromyces apiculatus (Ta) AT0115 and Clonostachys rosea (Cr) AAB0114 biocontrol species with PGP characteristics. In vitro studies have demonstrated both Ta and Cr are capable of reducing linear mycelial growth of G. boninense. Inoculation of individual Cr and Ta-as well as Cr+Ta consortium-induced a significant increment in leaf area and bole girth of oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At five months post-inoculation, shoot and root biomass, and nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium and boron) were significantly higher in Ta-inoculated seedlings compared to control treated with non-Ta-inoculated maize. Chlorophyll and carotenoids contents in rapidly growing oil-palm seedlings challenged with Cr, Ta or a combination of both were not negatively affected. Cr, Ta and Cr+Ta consortium treated seedlings had 4.9-60% BSR disease reduction compared to the untreated control. Co-inoculation of Cr and Ta resulted in increased BSR control efficiencies by 18-26% (compared with Cr only) and 48-55% (compared with Ta only). Collectively, Cr and Ta, either individually or in consortium showed potential as BSR biocontrol agents while also possess PGP traits in oil palm. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88027/1/ABSTRACT.pdf Goh, Yit Kheng and Marzuki, Nurul Fadhilah and Tuan Pa, Tuan Nur Fatihah and Goh, Teik Khiang and Kee, Zeng Seng and Goh, You Keng and Yusof, Mohd Termizi and Vujanovic, Vladimir and Goh, Kah Joo (2020) Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm. Microorganisms, 8 (8). 1138 - 1158. ISSN 2076-2607 https://www.mdpi.com/2076-2607/8/8/1138 10.3390/microorganisms8081138
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Basal stem rot (BSR) disease caused by Ganoderma boninense basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environmentally friendly biocontrol approaches in minimizing BSR disease. This study investigated the performance of two potential biocontrol agents (BCAs), AAT0115 and AAB0114 strains recovered from oil palm on suppression of BSR in planta, and also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated the two ascomycetous Talaromyces apiculatus (Ta) AT0115 and Clonostachys rosea (Cr) AAB0114 biocontrol species with PGP characteristics. In vitro studies have demonstrated both Ta and Cr are capable of reducing linear mycelial growth of G. boninense. Inoculation of individual Cr and Ta-as well as Cr+Ta consortium-induced a significant increment in leaf area and bole girth of oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At five months post-inoculation, shoot and root biomass, and nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium and boron) were significantly higher in Ta-inoculated seedlings compared to control treated with non-Ta-inoculated maize. Chlorophyll and carotenoids contents in rapidly growing oil-palm seedlings challenged with Cr, Ta or a combination of both were not negatively affected. Cr, Ta and Cr+Ta consortium treated seedlings had 4.9-60% BSR disease reduction compared to the untreated control. Co-inoculation of Cr and Ta resulted in increased BSR control efficiencies by 18-26% (compared with Cr only) and 48-55% (compared with Ta only). Collectively, Cr and Ta, either individually or in consortium showed potential as BSR biocontrol agents while also possess PGP traits in oil palm.
format Article
author Goh, Yit Kheng
Marzuki, Nurul Fadhilah
Tuan Pa, Tuan Nur Fatihah
Goh, Teik Khiang
Kee, Zeng Seng
Goh, You Keng
Yusof, Mohd Termizi
Vujanovic, Vladimir
Goh, Kah Joo
spellingShingle Goh, Yit Kheng
Marzuki, Nurul Fadhilah
Tuan Pa, Tuan Nur Fatihah
Goh, Teik Khiang
Kee, Zeng Seng
Goh, You Keng
Yusof, Mohd Termizi
Vujanovic, Vladimir
Goh, Kah Joo
Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm
author_facet Goh, Yit Kheng
Marzuki, Nurul Fadhilah
Tuan Pa, Tuan Nur Fatihah
Goh, Teik Khiang
Kee, Zeng Seng
Goh, You Keng
Yusof, Mohd Termizi
Vujanovic, Vladimir
Goh, Kah Joo
author_sort Goh, Yit Kheng
title Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm
title_short Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm
title_full Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm
title_fullStr Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm
title_full_unstemmed Biocontrol and plant-growth-promoting traits of Talaromyces apiculatus and Clonostachys rosea consortium against Ganoderma Basal Stem Rot disease of oil palm
title_sort biocontrol and plant-growth-promoting traits of talaromyces apiculatus and clonostachys rosea consortium against ganoderma basal stem rot disease of oil palm
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88027/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/88027/
https://www.mdpi.com/2076-2607/8/8/1138
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score 13.209306