Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does
Brucellosis has been regarded as the most widespread zoonotic disease. Infected animals have always been the source of human infection. Studies on the influence of stress on the pathological dynamics of this zoonotic disease are uncommon. This study aimed at evaluating serological responses, oxidati...
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my.upm.eprints.874702022-03-10T08:26:15Z http://psasir.upm.edu.my/id/eprint/87470/ Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does Polycarp, Nwunuji Tanko Mohd Yusoff, Sabri Emikpe, Benjamin Obukowho Bejo, Siti Khairani Salisi, Mohd Shahrom Brucellosis has been regarded as the most widespread zoonotic disease. Infected animals have always been the source of human infection. Studies on the influence of stress on the pathological dynamics of this zoonotic disease are uncommon. This study aimed at evaluating serological responses, oxidative status, and pathological derangements associated with Brucella melitensis infection in does treated with dexamethasone. Twelve does were divided into 4 groups designated A, B, C, and D with 3 animals each. Groups A and B were ocularly inoculated with 107 CFU of B. melitensis. From day 21 postinoculation (pi), dexamethasone was administered for 7 days at 2 mg/kg intramuscularly. Group A was further treated with resveratrol for 5 days from day 31 post-B. melitensis inoculation. Group C was inoculated with similar dose of B. melitensis ocularly while group D was inoculated normal saline. Blood samples were collected at regular intervals for PCR, serology, and oxidative stress analysis. The does were sacrificed at day 42 pi. Selected tissues were collected for immunohistochemistry and histopathological examination. Dexamethasone administration induced increase in molondialdehyde level, intensity of immunostaining, and pathological alterations while decreasing superoxide dismutase, and glutathione levels in group A compared to group B. Five-day treatment with resveratrol following dexamethasone administration was observed to significantly ameliorate pathological derangements compared to group A. While stress could exacerbate pathological alterations associated with B. melitensis infection, antioxidants are capable of mitigating the impact of stress associated diseases. Springer 2020-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87470/1/Resveratrol%20ameliorates.pdf Polycarp, Nwunuji Tanko and Mohd Yusoff, Sabri and Emikpe, Benjamin Obukowho and Bejo, Siti Khairani and Salisi, Mohd Shahrom (2020) Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does. Comparative Clinical Pathology, 29 (1). 255 - 266. ISSN 1618-565X https://link.springer.com/article/10.1007/s00580-019-03035-5 10.1007/s00580-019-03035-5 |
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Brucellosis has been regarded as the most widespread zoonotic disease. Infected animals have always been the source of human infection. Studies on the influence of stress on the pathological dynamics of this zoonotic disease are uncommon. This study aimed at evaluating serological responses, oxidative status, and pathological derangements associated with Brucella melitensis infection in does treated with dexamethasone. Twelve does were divided into 4 groups designated A, B, C, and D with 3 animals each. Groups A and B were ocularly inoculated with 107 CFU of B. melitensis. From day 21 postinoculation (pi), dexamethasone was administered for 7 days at 2 mg/kg intramuscularly. Group A was further treated with resveratrol for 5 days from day 31 post-B. melitensis inoculation. Group C was inoculated with similar dose of B. melitensis ocularly while group D was inoculated normal saline. Blood samples were collected at regular intervals for PCR, serology, and oxidative stress analysis. The does were sacrificed at day 42 pi. Selected tissues were collected for immunohistochemistry and histopathological examination. Dexamethasone administration induced increase in molondialdehyde level, intensity of immunostaining, and pathological alterations while decreasing superoxide dismutase, and glutathione levels in group A compared to group B. Five-day treatment with resveratrol following dexamethasone administration was observed to significantly ameliorate pathological derangements compared to group A. While stress could exacerbate pathological alterations associated with B. melitensis infection, antioxidants are capable of mitigating the impact of stress associated diseases. |
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Article |
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Polycarp, Nwunuji Tanko Mohd Yusoff, Sabri Emikpe, Benjamin Obukowho Bejo, Siti Khairani Salisi, Mohd Shahrom |
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Polycarp, Nwunuji Tanko Mohd Yusoff, Sabri Emikpe, Benjamin Obukowho Bejo, Siti Khairani Salisi, Mohd Shahrom Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does |
author_facet |
Polycarp, Nwunuji Tanko Mohd Yusoff, Sabri Emikpe, Benjamin Obukowho Bejo, Siti Khairani Salisi, Mohd Shahrom |
author_sort |
Polycarp, Nwunuji Tanko |
title |
Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does |
title_short |
Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does |
title_full |
Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does |
title_fullStr |
Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does |
title_full_unstemmed |
Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does |
title_sort |
resveratrol ameliorates pathophysiological changes associated with brucella melitensis infection in dexamethasone-treated does |
publisher |
Springer |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/87470/1/Resveratrol%20ameliorates.pdf http://psasir.upm.edu.my/id/eprint/87470/ https://link.springer.com/article/10.1007/s00580-019-03035-5 |
_version_ |
1728052816299163648 |
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13.160551 |