Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis

A novel peptide nuclide acid (PNA) electrochemical biosensor based on reduced graphene oxide (NH2-rGO)/2,2,6,6-tetramethylpiperidin-1-yl)oxyl nanocrystalline cellulose (TEMPO-NCC) for the detection of Mycobacterium tuberculosis (M. Tuberculosis) is described. In this study, the nanohybrid films NH2-...

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Main Authors: Mat Zaid, Mohd Hazani, Abdullah, Jaafar, Yusof, Nor Azah, Wasoh, Helmi, Sulaiman, Yusran, Md Noh, Mohd Fairulnizal, Issa, Rahizan
Format: Article
Published: Wiley 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87419/
https://www.hindawi.com/journals/js/2020/4051474/
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spelling my.upm.eprints.874192024-10-16T06:47:45Z http://psasir.upm.edu.my/id/eprint/87419/ Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis Mat Zaid, Mohd Hazani Abdullah, Jaafar Yusof, Nor Azah Wasoh, Helmi Sulaiman, Yusran Md Noh, Mohd Fairulnizal Issa, Rahizan A novel peptide nuclide acid (PNA) electrochemical biosensor based on reduced graphene oxide (NH2-rGO)/2,2,6,6-tetramethylpiperidin-1-yl)oxyl nanocrystalline cellulose (TEMPO-NCC) for the detection of Mycobacterium tuberculosis (M. Tuberculosis) is described. In this study, the nanohybrid films NH2-rGO/TEMPO-NCC were immobilized onto screen-printed carbon electrode (SPE) via a simple drop-coating method. The electrochemical characterization of the designed electrode was investigated using cyclic voltammetry (CV) and impedance spectroscopy (EIS). Meanwhile, the sensitivity and selectivity of the designed biosensor against M. tuberculosis were measured by the differential pulse voltammetry (DPV). The response of the PNA probe-modified (NH2-rGO)/TEMPO-NCC demonstrated that the fabricated biosensor was able to distinguish between complementary, noncomplementary, and one-base mismatch DNA sequences using methylene blue (MB) as the electrochemical indicator. The developed electrochemical biosensor exhibited a linear calibration curve in the concentration range of 1 × 10−8 M to 1 × 10−13 M with the limit of detection of 3.14 × 10−14 M. The developed electrochemical biosensor has also been tested with a polymerase chain reaction (PCR) product of M. tuberculosis DNA which has shown successful results in distinguishing between negative and positive samples of M. tuberculosis. Wiley 2020-01 Article PeerReviewed Mat Zaid, Mohd Hazani and Abdullah, Jaafar and Yusof, Nor Azah and Wasoh, Helmi and Sulaiman, Yusran and Md Noh, Mohd Fairulnizal and Issa, Rahizan (2020) Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis. Journal of Sensors, 2020 (1). pp. 1-11. ISSN 1687-725X; eISSN: 1687-7268 https://www.hindawi.com/journals/js/2020/4051474/ 10.1155/2020/4051474
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description A novel peptide nuclide acid (PNA) electrochemical biosensor based on reduced graphene oxide (NH2-rGO)/2,2,6,6-tetramethylpiperidin-1-yl)oxyl nanocrystalline cellulose (TEMPO-NCC) for the detection of Mycobacterium tuberculosis (M. Tuberculosis) is described. In this study, the nanohybrid films NH2-rGO/TEMPO-NCC were immobilized onto screen-printed carbon electrode (SPE) via a simple drop-coating method. The electrochemical characterization of the designed electrode was investigated using cyclic voltammetry (CV) and impedance spectroscopy (EIS). Meanwhile, the sensitivity and selectivity of the designed biosensor against M. tuberculosis were measured by the differential pulse voltammetry (DPV). The response of the PNA probe-modified (NH2-rGO)/TEMPO-NCC demonstrated that the fabricated biosensor was able to distinguish between complementary, noncomplementary, and one-base mismatch DNA sequences using methylene blue (MB) as the electrochemical indicator. The developed electrochemical biosensor exhibited a linear calibration curve in the concentration range of 1 × 10−8 M to 1 × 10−13 M with the limit of detection of 3.14 × 10−14 M. The developed electrochemical biosensor has also been tested with a polymerase chain reaction (PCR) product of M. tuberculosis DNA which has shown successful results in distinguishing between negative and positive samples of M. tuberculosis.
format Article
author Mat Zaid, Mohd Hazani
Abdullah, Jaafar
Yusof, Nor Azah
Wasoh, Helmi
Sulaiman, Yusran
Md Noh, Mohd Fairulnizal
Issa, Rahizan
spellingShingle Mat Zaid, Mohd Hazani
Abdullah, Jaafar
Yusof, Nor Azah
Wasoh, Helmi
Sulaiman, Yusran
Md Noh, Mohd Fairulnizal
Issa, Rahizan
Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis
author_facet Mat Zaid, Mohd Hazani
Abdullah, Jaafar
Yusof, Nor Azah
Wasoh, Helmi
Sulaiman, Yusran
Md Noh, Mohd Fairulnizal
Issa, Rahizan
author_sort Mat Zaid, Mohd Hazani
title Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis
title_short Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis
title_full Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis
title_fullStr Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis
title_full_unstemmed Reduced graphene oxide/TEMPO-nanocellulose nanohybrid based electrochemical biosensor for the determination of Mycobacterium tuberculosis
title_sort reduced graphene oxide/tempo-nanocellulose nanohybrid based electrochemical biosensor for the determination of mycobacterium tuberculosis
publisher Wiley
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87419/
https://www.hindawi.com/journals/js/2020/4051474/
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