Isolation and Characterisation of Bifidobacterium Spp. from Infant Stools
Infant faecal materials from different feeding regimes (breast fed, breast fed supplemented with mixed diet and bottle fed) were analysed for their distribution of microbes and isolation of bifidobacteria. The predominant bacteria in breast-fed infants after 23 weeks were streptococci and bifido...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8718/1/FSMB_1998_6_A.pdf http://psasir.upm.edu.my/id/eprint/8718/ |
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| Summary: | Infant faecal materials from different feeding regimes (breast fed,
breast fed supplemented with mixed diet and bottle fed) were analysed
for their distribution of microbes and isolation of bifidobacteria. The
predominant bacteria in breast-fed infants after 23 weeks were
streptococci and bifidobacteria. The predominant bacteria in breast-fed
supplemented with mixed diet after 24 weeks were clostridia,
streptococci and bifidobacteria. In the bottle fed infant faeces,
bifidobacteria was present in small numbers whereas lactobacilli were
one of the most predominant bacteria. 18 isolates of Bifidobacterium
in/antis and 10 isolates of Bifidobacterium breve were isolated and tested
for their antibacterial activity, survival in low pH conditions, and
adhesion to human colon carcinoma cell lines . Three isolates (B. in/antisC040225, B. breve F0526100 and B. breve G012048) exhibited good
antibacterial activity. These isolates inhibit the growth of Salmonella
enteritidis, Vibrio cholera, Escherichia coli, Bacillus cereus, Pseudomonas
aerugmosa and Listeria monocytogenes. The survival rate of
bifidobacteria in low pH condition was variable among isolates. All the
isolates tested showed good survival in control solution (pH 6.5). None
of the isolates tested was able to survive at pH 1.0 after 1 hour
incubation. However, at pH 2.0, five isolates (B. infantis D042022, B.
infantis F042466, B. infantis F0526100 and B. infantis G001204)
survived after 1h incubation but not after 2h incubation at this pH.
Four strains (B. infantis D04 2022, B. infantis F0606117, B. infantis
F041134 and B. infantis Z040845) possessed good survival at pH 3.0
where viable cells could still be detected after 3 hours incubation. One
of the most important criteria of probiotic micro-organism is the ability
to adhere and colonise to human intestinal epithelial cells in order to
prevent them from being flushed out by the peristaltic movement in the
gastrointestinal tract. B. infantis G001204 was found to adhere well to
HT29 cell lines in culture. Not all the bifidobacteria isolates tested
possessed all the characteristics of probiotic. |
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