Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.)
This study was conducted to determine the localisation of antigen in the gut of vaccinated and non-vaccinated red hybrid tilapia post-challenge via immunohistochemistry and scanning electron microscopy. All groups (1, 2, 3, and 4) were vaccinated orally with feed-based killed whole-cells of Streptoc...
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my.upm.eprints.865872021-09-28T23:20:18Z http://psasir.upm.edu.my/id/eprint/86587/ Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) M., Hayat M. Y., Sabri Abdul Razak, Intan Shameha Md Yasin, Ina Salwany Thompson, Kim D. This study was conducted to determine the localisation of antigen in the gut of vaccinated and non-vaccinated red hybrid tilapia post-challenge via immunohistochemistry and scanning electron microscopy. All groups (1, 2, 3, and 4) were vaccinated orally with feed-based killed whole-cells of Streptococcus iniae (FKV). Group 1 was fed with the FKV for 3 days consecutively only on day 0; group 2 was fed with the FKV for 3 days consecutively on day 0 and booster on day 14; group 3 was fed with the FKV for 3 days consecutively on day 0, followed by a booster on day 14 and 21; and group 4 was fed with the FKV for 5 days consecutively on day 0, followed by a booster on day 14 and 21. The remaining groups, group 5 (negative control group) and group 6 (positive control group), only fed with a commercial feed. Five fish from each group were sacrificed on a weekly basis for the entire 6 weeks for a collection of fresh small intestinal samples including post-challenge starting from 4 to 72 h. Immunohistochemical staining was conducted using the avidin-biotin-peroxidase complex method. Scanning electron microscopy was also used to view the hindgut samples from each group. Positive control group recorded significantly the highest (p < 0.05) antigen positivity, followed by groups 1 and 2, meanwhile, lowest positivity in groups 3 and 4 at 12 h, 24 h, and 72 h, respectively. Immunohistochemistry and scanning electron microscopy showed the presence of antigens in red hybrid tilapia hindgut, protruding in goblet cells, gut-associated lymphoid tissues, and villi dome epithelium in different hours of post-challenge. Significantly, boosted group 4 with a low number of antigens in 72 h showed the stimulation and irregular projection of M-cells with variable morphology and antigen distribution pattern. Springer 2020-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86587/1/Localisation%20of%20antigens%20.pdf M., Hayat and M. Y., Sabri and Abdul Razak, Intan Shameha and Md Yasin, Ina Salwany and Thompson, Kim D. (2020) Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.). Aquaculture International, 28. pp. 1739-1752. ISSN 0967-6120; ESSN: 1573-143X https://link.springer.com/article/10.1007/s10499-020-00554-9 10.1007/s10499-020-00554-9 |
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This study was conducted to determine the localisation of antigen in the gut of vaccinated and non-vaccinated red hybrid tilapia post-challenge via immunohistochemistry and scanning electron microscopy. All groups (1, 2, 3, and 4) were vaccinated orally with feed-based killed whole-cells of Streptococcus iniae (FKV). Group 1 was fed with the FKV for 3 days consecutively only on day 0; group 2 was fed with the FKV for 3 days consecutively on day 0 and booster on day 14; group 3 was fed with the FKV for 3 days consecutively on day 0, followed by a booster on day 14 and 21; and group 4 was fed with the FKV for 5 days consecutively on day 0, followed by a booster on day 14 and 21. The remaining groups, group 5 (negative control group) and group 6 (positive control group), only fed with a commercial feed. Five fish from each group were sacrificed on a weekly basis for the entire 6 weeks for a collection of fresh small intestinal samples including post-challenge starting from 4 to 72 h. Immunohistochemical staining was conducted using the avidin-biotin-peroxidase complex method. Scanning electron microscopy was also used to view the hindgut samples from each group. Positive control group recorded significantly the highest (p < 0.05) antigen positivity, followed by groups 1 and 2, meanwhile, lowest positivity in groups 3 and 4 at 12 h, 24 h, and 72 h, respectively. Immunohistochemistry and scanning electron microscopy showed the presence of antigens in red hybrid tilapia hindgut, protruding in goblet cells, gut-associated lymphoid tissues, and villi dome epithelium in different hours of post-challenge. Significantly, boosted group 4 with a low number of antigens in 72 h showed the stimulation and irregular projection of M-cells with variable morphology and antigen distribution pattern. |
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M., Hayat M. Y., Sabri Abdul Razak, Intan Shameha Md Yasin, Ina Salwany Thompson, Kim D. |
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M., Hayat M. Y., Sabri Abdul Razak, Intan Shameha Md Yasin, Ina Salwany Thompson, Kim D. Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) |
author_facet |
M., Hayat M. Y., Sabri Abdul Razak, Intan Shameha Md Yasin, Ina Salwany Thompson, Kim D. |
author_sort |
M., Hayat |
title |
Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) |
title_short |
Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) |
title_full |
Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) |
title_fullStr |
Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) |
title_full_unstemmed |
Localisation of antigens in the gut post-challenge with Streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (Oreochromis sp.) |
title_sort |
localisation of antigens in the gut post-challenge with streptococcus iniae in vaccinated and non-vaccinated red hybrid tilapia (oreochromis sp.) |
publisher |
Springer |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/86587/1/Localisation%20of%20antigens%20.pdf http://psasir.upm.edu.my/id/eprint/86587/ https://link.springer.com/article/10.1007/s10499-020-00554-9 |
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1712286742461022208 |
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13.160551 |