Limited expression of non-integrating CpG-free plasmid is associated with increased nucleosome enrichment

• Main Authors: Habib, Omar, M. Sakri, Rozita, Ghazalli, Nadiah, Chau, De Ming, Ling, King Hwa, Abdullah, Syahril
• Format: Article
• Language: English
• Published: Public Library of Science 2020
• Online Access: http://psasir.upm.edu.my/id/eprint/86578/1/Limited%20expression%20of%20non-integrating%20.pdf; http://psasir.upm.edu.my/id/eprint/86578/; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244386

CpG-free pDNA was reported to facilitate sustained transgene expression with minimal inflammation in vivo as compared to CpG-containing pDNA. However, the expression potential and impact of CpG-free pDNA in in vitro model have never been described. Hence, in this study, we analyzed the transgene exp...