Characterization of bacteriophages for E. coli causing colibacilosis in chicken

• Main Author: Nur Azami, Nur Athirah
• Format: Project Paper Report
• Language: English
• Published: 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/85126/1/FBSB%202015%20122%20-%20IR.pdf; http://psasir.upm.edu.my/id/eprint/85126/

The emergence of pathogenic bacteria resistant towards most of the antimicrobial agents has risen up the bacteriophage therapy research through out the world. Rather then using antibiotics, it is suggested that the bacterial infection is treated by the administration of bacteriophages. The research was performed in order to characterise the bacteriophages against the pathogenic bacteria Escherichia coli (E. coli) that have been identified as the causing agent of the colibacilosis in chicken. The host bacteria of the bacteriophages were E. coli strains 712X, 141 1E, 141 2E, and 141 3E. All of these E. coli strains were serotype O1:K1 which is causative agent for colibacillosis in chicken. There are two bacteriophages that are been characterised which are ØMS1 and ØMS2. All the bacteriophages were characterised using physiological characterisation. Both bacteriophages show their lytic ability toward E. coli strains 7I2X only. It is shown that ØMS1 is better in comparison of ØMS2. Both ØMS1 and ØMS2 demonstrated an optimum multiplicity of infection (MOI) of 0.01 and 0.1 respectively. Based on adsorption rate, ØMS1 reach it maximum value (99.8%) at 8 minutes while ØMS2 reach it maximum value (99.6%) at 10 minutes. In the single step growth curve of the bacteriophages, ØMS1 have longer latent period (25 min) compare to ØMS2 (15 min), with higher burst size of 200 bacteriophage particles per infected cell and 175 bacteriophage particles per infected cell respectively. Both ØMS1 and ØMS2 are stable in temperature range of 37-40 oC with pH range of 6-9.