Isolation and analysis of rhlC and rhlR from rhamnolipid producing bacterium, Pseudomonas aeruginosa strain S5

• Main Author: Mohd Sidik, Noor Hayati
• Format: Project Paper Report
• Language: English
• Published: 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/85051/1/FBSB%202015%20117%20-%20IR.pdf; http://psasir.upm.edu.my/id/eprint/85051/

Rhamnolipids are biomolecules with a huge potential in leading global surfactant market, which find applications in various kind of industry in demand for emulsifying and solubilizing agent including food industry, pharmaceutical industry, and petroleum industry. Rhamnolipids are being majorly produced by microorganism, particularly, Pseudomonas aeruginosa. This study aims to test rhamnolipid production in Pseudomonas aeruginosa strain S5 and to analyze the genes involve for the rhamnolipids biosynthesis which are rhlC and rhlR that code for rhamnosyltransferase 2 and transcription regulator respectively. Qualitative test on cetyltrimethyl ammonium bromide agar and quantitative tests by emulsification index valued 37.44 %, display capabilities of Pseudomonas aeruginosa strain S5 in producing rhamnolipid. In molecular detection of rhamnolipid production, BLAST result shows high similarity between both rhlC and rhlR sequences, and sequence available in database. The information from the gene analysis could facilitate the understanding on the rhamnolipid biosynthesis in Pseudomonas aeruginosa strain S5, hence can be further used in genetic modulation in order to meet future prospects.