Isolation And Characterization Of Disease And Stress Related Genes From Muskmelon (Cucumis Melo L.)
Soil salinity and attack by pathogen a re the major abiotic stresses in plant agriculture worldwide. Past efforts to improve plant tolerance to osmotic stress and pathogen attack through breeding and genetic engineering have shown limited success owing to the genetic complexity of stress response...
Saved in:
Main Author: | |
---|---|
Format: | Thesis |
Language: | English English |
Published: |
2002
|
Subjects: | |
Online Access: | http://psasir.upm.edu.my/id/eprint/8472/1/FSMB_2002_10_IR.pdf http://psasir.upm.edu.my/id/eprint/8472/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Soil salinity and attack by pathogen a re the major abiotic stresses in
plant agriculture worldwide. Past efforts to improve plant tolerance to osmotic
stress and pathogen attack through breeding and genetic engineering have
shown limited success owing to the genetic complexity of stress responses.
Large-scale partial sequencing of expressed sequence tags (ESTs)
towards cataloging and categorizing genetically abiotic stress responses can
assist a means for the rapid discovery of stress-specific genes. A combination
of two types of abiotic stresses (200mM of NaCI and 14 min of 254nm UV-irradiation) were applied to muskmelon seedlings prior to the isolation of RNA
for cDNA library construction. Cold plaque hybridisation using non-induced
cDNA as probe to screen the stress-induced cDNA library for stress and
disease-related genes was performed. A total of 550 ESTs were generated
from a stress-induced cDNA library with a cDNA insert size ranging between
500-2500bp. Classification of the ESTs based on their designated functions showed
that the majority of isolated genes were involved in salt tolerance and disease
resistance. The results showed that the generation of stress-induced ESTs by
partial sequencing of random cDNA clones along with expression analysis are
efficient approach to identify isolated genes that a re responsible for salt
tolerance and disease resistance on a large scale. We believe our dbEST
and the associated DNA materials would be a useful resource to scientists
engaged in studies on stress-tolerance and disease resistance.
MYB proteins a re known to regulate different branches of flavonoid
metabolism in plants in response to wounding, UV irradiation and ethephon
treatment. Their role as transcriptional regulators in response to stress
treatment and pathogen attack also has been reported (Urao et al., 1993; Abe
et al., 1997). A 279bp partial MYB-related protein was also isolated by an RT-PCR
method using the degenerate primers. At the same time, the double
induced stress-related muskmelon cDNA library of salt and UV irradiation
provide a good source to study the gene expression and regulatory of MYB
gene in relation to the stress and UV treatment. Due to the high similarity of
the partial MYB sequence to the cloning vector, p Bluescript SK(+), screening
of the full length MYB gene was unsuccessful. |
---|