Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation

The aim of this study was to evaluate the effect of supplementing Tris (Tr) and Bioxcell (Bx) extenders with edible-bird’s nest (EBN) on the quality of bull sperm upon cryopreservation. A total of 12 semen samples were collected from mature bulls by electroejaculation. A total of 12 semen samples we...

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Main Author: Yusop, Dayang Rakhmioktaleawatty
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/83362/1/FPV%202016%2080%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83362/
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spelling my.upm.eprints.833622020-09-17T01:51:27Z http://psasir.upm.edu.my/id/eprint/83362/ Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation Yusop, Dayang Rakhmioktaleawatty The aim of this study was to evaluate the effect of supplementing Tris (Tr) and Bioxcell (Bx) extenders with edible-bird’s nest (EBN) on the quality of bull sperm upon cryopreservation. A total of 12 semen samples were collected from mature bulls by electroejaculation. A total of 12 semen samples were collected from mature bulls by electroejaculation. The semen samples were evaluated both freshly and after cryopreservation for quality based on % sperm general and progressive motility (under a microscope), viability and abnormal morphology (using eosin-nigrosin stain). The fresh samples were then diluted and extended using the two extenders containing 0% (control), 0.03%, 0.06%, and 0.12% of EBN. Chilled at 4°C for 3 hours before packaged into 0.25 mL straws and frozen into liquid nitrogen (-196°C) for 48 hours. Results for both extenders revealed insignificant differences (P>0.05) in all parameters between the different EBN treatment groups and control. Although not significant, 0.12% EBN in both extenders showed the lowest % abnormality, close to the fresh sample reading. Moreover, Bx tended to show reduced % abnormality than Tr for the same EBN treatments, implying more protective role played. In conclusion, EBN concentrations used in this study do not significantly improve sperm quality after freezing. Further research should be carried out with increased sample size and dosage of EBN in order to clarify the minimal improvement observed for parameters such as sperm abnormality. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/83362/1/FPV%202016%2080%20-%20IR.pdf Yusop, Dayang Rakhmioktaleawatty (2016) Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of this study was to evaluate the effect of supplementing Tris (Tr) and Bioxcell (Bx) extenders with edible-bird’s nest (EBN) on the quality of bull sperm upon cryopreservation. A total of 12 semen samples were collected from mature bulls by electroejaculation. A total of 12 semen samples were collected from mature bulls by electroejaculation. The semen samples were evaluated both freshly and after cryopreservation for quality based on % sperm general and progressive motility (under a microscope), viability and abnormal morphology (using eosin-nigrosin stain). The fresh samples were then diluted and extended using the two extenders containing 0% (control), 0.03%, 0.06%, and 0.12% of EBN. Chilled at 4°C for 3 hours before packaged into 0.25 mL straws and frozen into liquid nitrogen (-196°C) for 48 hours. Results for both extenders revealed insignificant differences (P>0.05) in all parameters between the different EBN treatment groups and control. Although not significant, 0.12% EBN in both extenders showed the lowest % abnormality, close to the fresh sample reading. Moreover, Bx tended to show reduced % abnormality than Tr for the same EBN treatments, implying more protective role played. In conclusion, EBN concentrations used in this study do not significantly improve sperm quality after freezing. Further research should be carried out with increased sample size and dosage of EBN in order to clarify the minimal improvement observed for parameters such as sperm abnormality.
format Project Paper Report
author Yusop, Dayang Rakhmioktaleawatty
spellingShingle Yusop, Dayang Rakhmioktaleawatty
Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation
author_facet Yusop, Dayang Rakhmioktaleawatty
author_sort Yusop, Dayang Rakhmioktaleawatty
title Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation
title_short Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation
title_full Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation
title_fullStr Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation
title_full_unstemmed Effect of edible-bird's nest in Tris and Bioxcell extenders on bull semen cryopreservation
title_sort effect of edible-bird's nest in tris and bioxcell extenders on bull semen cryopreservation
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/83362/1/FPV%202016%2080%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83362/
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score 13.18916