Cover Image

The influence of serum‐supplemented culture media in a transwell migration assay

Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FB...

Full description

Saved in:
Bibliographic Details
Main Authors: Omar Zaki, Siti Sarah, Kanesan, Livashini, Leong, Ming Yeh Danielle, Vidyadaran, Sharmili
Format: Article
Language:English
Published: Wiley 2019
Online Access:http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf
http://psasir.upm.edu.my/id/eprint/82524/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.82524
record_format eprints
spelling my.upm.eprints.825242020-10-16T20:20:41Z http://psasir.upm.edu.my/id/eprint/82524/ The influence of serum‐supplemented culture media in a transwell migration assay Omar Zaki, Siti Sarah Kanesan, Livashini Leong, Ming Yeh Danielle Vidyadaran, Sharmili Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used. Wiley 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf Omar Zaki, Siti Sarah and Kanesan, Livashini and Leong, Ming Yeh Danielle and Vidyadaran, Sharmili (2019) The influence of serum‐supplemented culture media in a transwell migration assay. Cell Biology International, 43 (10). pp. 1201-1204. ISSN 1065-6995; ESSN: 1095-8355 10.1002/cbin.11122
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.
format Article
author Omar Zaki, Siti Sarah
Kanesan, Livashini
Leong, Ming Yeh Danielle
Vidyadaran, Sharmili
spellingShingle Omar Zaki, Siti Sarah
Kanesan, Livashini
Leong, Ming Yeh Danielle
Vidyadaran, Sharmili
The influence of serum‐supplemented culture media in a transwell migration assay
author_facet Omar Zaki, Siti Sarah
Kanesan, Livashini
Leong, Ming Yeh Danielle
Vidyadaran, Sharmili
author_sort Omar Zaki, Siti Sarah
title The influence of serum‐supplemented culture media in a transwell migration assay
title_short The influence of serum‐supplemented culture media in a transwell migration assay
title_full The influence of serum‐supplemented culture media in a transwell migration assay
title_fullStr The influence of serum‐supplemented culture media in a transwell migration assay
title_full_unstemmed The influence of serum‐supplemented culture media in a transwell migration assay
title_sort influence of serum‐supplemented culture media in a transwell migration assay
publisher Wiley
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf
http://psasir.upm.edu.my/id/eprint/82524/
_version_ 1681490856103641088
score 13.160551

Similar Items