The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells

• Main Authors: Sung, Tzu Cheng, Yang, Jia Sin, Yeh, Chih Chen, Liu, Ya Chu, Jiang, Yi Peng, Lu, Ming We, Ling, Qing Dong, Kumar, S. Suresh, Chang, Yung, Umezawa, Akihiro, Chen, Hao, Higuchi, Akon
• Format: Article
• Language: English
• Published: Elsevier 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/82380/1/The%20design%20of%20a%20thermoresponsive.pdf; http://psasir.upm.edu.my/id/eprint/82380/; https://www.sciencedirect.com/science/article/abs/pii/S0142961219305101

Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the th...