Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein
An optical sensor for the dengue virus (DENV) E-protein based on cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) thin film was successfully developed. A specific monoclonal antibodies (IgM) were covalently attached to CdS-NH2GO via EDC/NHS coupling to sen...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82088/1/Sensitive%20surface%20plasmon%20resonance%20.pdf http://psasir.upm.edu.my/id/eprint/82088/ https://www.sciencedirect.com/science/article/abs/pii/S0030399218306819 |
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| Summary: | An optical sensor for the dengue virus (DENV) E-protein based on cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) thin film was successfully developed. A specific monoclonal antibodies (IgM) were covalently attached to CdS-NH2GO via EDC/NHS coupling to sense targeted E-proteins. The SPR sensor exhibited an excellent detection limit (0.001 nM/1 pM) with sensitivity of 5.49° nM−1 for the detection of DENV E-protein. The binding affinity, as well as the performance of the Au/CdS-NH2GO/EDC-NHS/IgM film, was successfully obtained at 486.54 nM−1 in detecting DENV E-proteins. These results indicated that the Au/CdS-NH2GO/EDC-NHS/IgM film shows high potential sensitive and stronger binding towards DENV E-protein. |
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