Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001

• Main Authors: Yustinah, Nurul Hidayat, Alamsyah, Rizal, Roslan, Ahmad Muhaimin, Hermansyah, Heri, Gozan, Misri
• Format: Article
• Language: English
• Published: Elsevier 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/81850/1/Production%20of%20polyhydroxybutyrate%20from%20oil%20palm%20empty%20fruit%20bunch%20%28OPEFB%29%20hydrolysates%20by%20Bacillus%20cereus%20suaeda%20B-001.pdf; http://psasir.upm.edu.my/id/eprint/81850/; https://reader.elsevier.com/reader/sd/pii/S1878818118304316?token=B7DB0EA31223FEDF7C354F8CDE5E1B1342EC668F3FEEC2B12FD71EE33E08286E8FABBFE0FF28916F62531728455BB531

Polyhydroxybutyrate (PHB) is a biodegradable polymer accumulated in intracellular granules by numerousbacteria. Its physical and chemical characteristics are like those of petrochemical plastics. PHB is producedmainly by gram-negative bacteria such asRalstonia eutropha, which have lipopolysaccharides that co-purify withthe PHB and cause immunogenic reactions, limiting their use for biomedical applications. PHB produced fromgram-positive bacteria such asBacillus spp.do not have lipopolysaccharides, which makes it suitable for bio-medical application. The aim of this work was to evaluate the ability ofBacillus cereus suaedaB-001 to accu-mulate PHB using oil palm empty fruit bunch (OPEFB) hydrolysate as the sole carbon source, comparing it tocommercial glucose as the control. OPEFB was chemically pre-treated using an acid-hydrolysed process bysulphuric acid and neutralized by a NaOH solution to obtain reducing sugars. PHB biopolyester accumulated to43.1% of cell dry weight with glucose at 15 g/L as the sole carbon source, and PHB accumulated to 55.4% of celldry weight using OPEFB hydrolysates at 20 g/L. The conversion of OPEFB acid hydrolysates to PHB using thegram-positive bacteriaBacillus cereus suaedaB-001 has not been reported.