Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury
Lung cancer is the leading cause of cancer related deaths worldwide with about 40% occurring in developing countries. The two varieties of Momordica charantia, which are Chinese and Indian bitter melon, have been subjected to antiproliferative activity in human non-small cell lung cells A549. The A5...
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2019
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my.upm.eprints.814182021-06-13T23:25:35Z http://psasir.upm.edu.my/id/eprint/81418/ Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury Thiagarajan, Siroshini Arapoc, Daryl Jesus Shafie, Nurul Husna Yong, Yoke Keong Bahari, Hasnah Adam, Zainah Ei, Thandar Lung cancer is the leading cause of cancer related deaths worldwide with about 40% occurring in developing countries. The two varieties of Momordica charantia, which are Chinese and Indian bitter melon, have been subjected to antiproliferative activity in human non-small cell lung cells A549. The A549 cells were treated with hot and cold aqueous extraction for both the bitter melon varieties, and the antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptotic mechanism of action on A549 human lung cancer cells was evaluated first morphologically using Hoechst 33358, and cytoskeleton staining using Filamentous-actin (F-actin) cytoskeleton FICT and DAPI followed by caspase-3/7, reactive oxygen species (ROS), and p53 activity. Chinese hot aqueous extraction (CHA) exhibited potent antiproliferative activity against A549 human lung cancer cells. The morphological analysis of mitochondria destruction and the derangement of cytoskeleton showed apoptosis-inducing activity. CHA increased the caspase-3/7 activity by 1.6-fold and the ROS activity by 5-fold. Flow cytometric analysis revealed 34.5% of apoptotic cells significantly (p<0.05) compared to cisplatin-treated A549 human cancer cells. CHA is suggested to induce apoptosis due to their rich bioactive chemical constituents. These findings suggest that the antiproliferative effect of CHA was due to apoptosis via ROS-mediated mitochondria injury. Hindawi 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81418/1/MOMO.pdf Thiagarajan, Siroshini and Arapoc, Daryl Jesus and Shafie, Nurul Husna and Yong, Yoke Keong and Bahari, Hasnah and Adam, Zainah and Ei, Thandar (2019) Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury. Evidence-based Complementary and Alternative Medicine, 2019. art. no. 2821597. pp. 1-9. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2019/2821597/ 10.1155/2019/2821597 |
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Lung cancer is the leading cause of cancer related deaths worldwide with about 40% occurring in developing countries. The two varieties of Momordica charantia, which are Chinese and Indian bitter melon, have been subjected to antiproliferative activity in human non-small cell lung cells A549. The A549 cells were treated with hot and cold aqueous extraction for both the bitter melon varieties, and the antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptotic mechanism of action on A549 human lung cancer cells was evaluated first morphologically using Hoechst 33358, and cytoskeleton staining using Filamentous-actin (F-actin) cytoskeleton FICT and DAPI followed by caspase-3/7, reactive oxygen species (ROS), and p53 activity. Chinese hot aqueous extraction (CHA) exhibited potent antiproliferative activity against A549 human lung cancer cells. The morphological analysis of mitochondria destruction and the derangement of cytoskeleton showed apoptosis-inducing activity. CHA increased the caspase-3/7 activity by 1.6-fold and the ROS activity by 5-fold. Flow cytometric analysis revealed 34.5% of apoptotic cells significantly (p<0.05) compared to cisplatin-treated A549 human cancer cells. CHA is suggested to induce apoptosis due to their rich bioactive chemical constituents. These findings suggest that the antiproliferative effect of CHA was due to apoptosis via ROS-mediated mitochondria injury. |
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Article |
author |
Thiagarajan, Siroshini Arapoc, Daryl Jesus Shafie, Nurul Husna Yong, Yoke Keong Bahari, Hasnah Adam, Zainah Ei, Thandar |
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Thiagarajan, Siroshini Arapoc, Daryl Jesus Shafie, Nurul Husna Yong, Yoke Keong Bahari, Hasnah Adam, Zainah Ei, Thandar Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury |
author_facet |
Thiagarajan, Siroshini Arapoc, Daryl Jesus Shafie, Nurul Husna Yong, Yoke Keong Bahari, Hasnah Adam, Zainah Ei, Thandar |
author_sort |
Thiagarajan, Siroshini |
title |
Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury |
title_short |
Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury |
title_full |
Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury |
title_fullStr |
Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury |
title_full_unstemmed |
Momordica charantia (Indian and Chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line A549 via ROS-mediated mitochodria injury |
title_sort |
momordica charantia (indian and chinese bitter melon) extracts inducing apoptosis in human lung cancer cell line a549 via ros-mediated mitochodria injury |
publisher |
Hindawi |
publishDate |
2019 |
url |
http://psasir.upm.edu.my/id/eprint/81418/1/MOMO.pdf http://psasir.upm.edu.my/id/eprint/81418/ https://www.hindawi.com/journals/ecam/2019/2821597/ |
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1703962162532188160 |
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13.18916 |