Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability
Malaysian Tualang honey (TH) is a known therapeutic honey extracted from the honeycombs of the Tualang tree (Koompassia excelsa) and has been reported for its antioxidant, anti-inflammatory, antiproliferative, and wound healing properties. However, the possible vascular protective effect of TH agai...
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2019
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my.upm.eprints.812722021-06-14T23:35:24Z http://psasir.upm.edu.my/id/eprint/81272/ Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability Devasvaran, Kogilavanee Tan, Jun Jie Theng, Ng Theng Lai, Yen Fong Yong, Yoke Keong Malaysian Tualang honey (TH) is a known therapeutic honey extracted from the honeycombs of the Tualang tree (Koompassia excelsa) and has been reported for its antioxidant, anti-inflammatory, antiproliferative, and wound healing properties. However, the possible vascular protective effect of TH against oxidative stress remains unclear. In this study, the effects of TH on hydrogen peroxide- (H2O2-) elicited vascular hyperpermeability in human umbilical vein endothelial cells (HUVECs) and Balb/c mice were evaluated. Our data showed that TH concentrations ranging from 0.01% to 1.00% showed no cytotoxic effect to HUVECs. Induction with 0.5 mM H2O2 was found to increase HUVEC permeability, but the effect was significantly reversed attenuated by TH (p < 0 05), of which the permeability with the highest inhibition peaked at 0.1%. In Balb/c mice, TH (0.5 g/kg-1.5 g/kg) significantly (p < 0 05) reduced H2O2 (0.3%)-induced albumin-bound Evans blue leak, in a dose-dependent manner. Immunofluorescence staining confirmed that TH reduced actin stress fiber formation while increasing cortical actin formation and colocalization of caveolin-1 and β-catenin in HUVECs. Signaling studies showed that HUVECs pretreated with TH significantly (p < 0 05) decreased intracellular calcium release, while sustaining the level of cAMP when challenged with H2O2. These results suggested that TH could inhibit H2O2-induced vascular hyperpermeability in vitro and in vivo by suppression of adherence junction protein redistribution via calcium and cAMP, which could have a therapeutic potential for diseases related to the increase of both oxidant and vascular permeability. Hindawi Publishing Corporation 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81272/1/Malaysian%20Tualang%20honey%20.pdf Devasvaran, Kogilavanee and Tan, Jun Jie and Theng, Ng Theng and Lai, Yen Fong and Yong, Yoke Keong (2019) Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability. Oxidative Medicine and Cellular Longevity, 2019. art. no. 1202676. pp. 1-10. ISSN 1942-0900; ESSN: 1942-0994 https://www.hindawi.com/journals/omcl/2019/1202676/ 10.1155/2019/1202676 |
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Malaysian Tualang honey (TH) is a known therapeutic honey extracted from the honeycombs of the Tualang tree (Koompassia
excelsa) and has been reported for its antioxidant, anti-inflammatory, antiproliferative, and wound healing properties. However, the possible vascular protective effect of TH against oxidative stress remains unclear. In this study, the effects of TH on hydrogen peroxide- (H2O2-) elicited vascular hyperpermeability in human umbilical vein endothelial cells (HUVECs) and Balb/c mice were evaluated. Our data showed that TH concentrations ranging from 0.01% to 1.00% showed no cytotoxic effect to HUVECs. Induction with 0.5 mM H2O2 was found to increase HUVEC permeability, but the effect was significantly reversed attenuated by TH (p < 0 05), of which the permeability with the highest inhibition peaked at 0.1%. In Balb/c mice, TH (0.5 g/kg-1.5 g/kg) significantly (p < 0 05) reduced H2O2 (0.3%)-induced albumin-bound Evans blue leak, in a dose-dependent manner. Immunofluorescence staining confirmed that TH reduced actin stress fiber formation while
increasing cortical actin formation and colocalization of caveolin-1 and β-catenin in HUVECs. Signaling studies showed that HUVECs pretreated with TH significantly (p < 0 05) decreased intracellular calcium release, while sustaining the level of cAMP when challenged with H2O2. These results suggested that TH could inhibit H2O2-induced vascular hyperpermeability in vitro and in vivo by suppression of adherence junction protein redistribution via calcium and cAMP, which could have a therapeutic potential for diseases related to the increase of both oxidant and vascular permeability. |
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Article |
author |
Devasvaran, Kogilavanee Tan, Jun Jie Theng, Ng Theng Lai, Yen Fong Yong, Yoke Keong |
spellingShingle |
Devasvaran, Kogilavanee Tan, Jun Jie Theng, Ng Theng Lai, Yen Fong Yong, Yoke Keong Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
author_facet |
Devasvaran, Kogilavanee Tan, Jun Jie Theng, Ng Theng Lai, Yen Fong Yong, Yoke Keong |
author_sort |
Devasvaran, Kogilavanee |
title |
Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
title_short |
Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
title_full |
Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
title_fullStr |
Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
title_full_unstemmed |
Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
title_sort |
malaysian tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability |
publisher |
Hindawi Publishing Corporation |
publishDate |
2019 |
url |
http://psasir.upm.edu.my/id/eprint/81272/1/Malaysian%20Tualang%20honey%20.pdf http://psasir.upm.edu.my/id/eprint/81272/ https://www.hindawi.com/journals/omcl/2019/1202676/ |
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1703962154409918464 |
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13.188404 |