Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

• Main Authors: Mustapha Kamil, Yasmin, Al-Rekabi, Sura Hmoud, Yaacob, Mohd Hanif, Syahir, Amir, Hui, Yee Chee, Mahdi, Mohd Adzir, Abu Bakar, Muhammad Hafiz
• Format: Article
• Language: English
• Published: Nature Publishing Group 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/81110/1/DENGUE.pdf; http://psasir.upm.edu.my/id/eprint/81110/; https://www.nature.com/articles/s41598-019-49891-7.pdf

The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent feld that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; antiDENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53nm/nM and 1 pM, respectively with Kd=1.02×10−10M.