Improved extracellular secretion of β-cyclodextrin glycosyltransferase from Escherichia coli by glycine supplementation without apparent cell lysis

• Main Authors: Nik Pa, Nik Ida Mardiana, Abd. Aziz, Suraini, Ibrahim, Mohamad Faizal, Mohammed Alitheen, Noorjahan Banu, Ramli, Norhayati
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/80183/1/Improved%20extracellular%20secretion%20of%20%CE%B2-cyclodextrin%20glycosyltransferase%20from%20Escherichia%20coli%20by%20glycine%20supplementation%20without%20apparent%20cell%20lysis.pdf; http://psasir.upm.edu.my/id/eprint/80183/; https://www.researchgate.net/publication/333815135_Improved_extracellular_secretion_of_b-cyclodextrin_glycosyltransferase_from_Escherichia_coli_by_glycine_supplementation_without_apparent_cell_lysis

The use of an effective inducer feeding strategy without causing cell lysis presents significant advantage to enhance the secretion of an enzyme to the culture medium of Escherichia coli. The cgt gene encoding β-cyclodextrin glycosyltransferase (β-CGTase) was cloned into pQE30xa as an N-terminal His...