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Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms

Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions....

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Main Authors: A. H., Muhamad Afiq, Shaharuddin, Noor Azmi, R., Zubaidah
Format: Article
Language:English
Published: Malaysian Palm Oil Board 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80136/1/Identification%20of%20Reliable%20Reference%20Genes%20for%20Gene%20Expression%20Studies%20of%20Oil%20Palm%20Plantlets%20using%20NormFinder%20and%20BestKeeper%20Algorithms.pdf
http://psasir.upm.edu.my/id/eprint/80136/
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spelling my.upm.eprints.801362020-09-23T08:04:59Z http://psasir.upm.edu.my/id/eprint/80136/ Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms A. H., Muhamad Afiq Shaharuddin, Noor Azmi R., Zubaidah Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions. However, the accuracy and reliability of qRT-PCR to study gene expression demand the use of species-specific reference genes for normalisation. Therefore, appropriate reference genes for qRT-PCR need to be determined for oil palm ( Elaeis guineensis) tissues. In this study, six candidate reference genes namely actin, Cyp2, GRAS, EgEfa1, F-box, and GLU were examined in leaf, node, internode, root, cabbage, shoot primordial and basal stem tissues of two-year old clonal oil palms. Two statistical algorithms, NormFinder and BestKeeper verified the stability of GRAS and Cyp2 reference genes in the oil palm tissues tested. The combination of Cyp2 and GRAS reference genes are recommended for gene expression normalisation profiles in nurserystage oil palm plantlets. Malaysian Palm Oil Board 2019 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/80136/1/Identification%20of%20Reliable%20Reference%20Genes%20for%20Gene%20Expression%20Studies%20of%20Oil%20Palm%20Plantlets%20using%20NormFinder%20and%20BestKeeper%20Algorithms.pdf A. H., Muhamad Afiq and Shaharuddin, Noor Azmi and R., Zubaidah (2019) Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms. Journal of Oil Palm Research, 31 (2). pp. 1-8. ISSN 1511-2780 10.21894/jopr.2019.0010
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions. However, the accuracy and reliability of qRT-PCR to study gene expression demand the use of species-specific reference genes for normalisation. Therefore, appropriate reference genes for qRT-PCR need to be determined for oil palm ( Elaeis guineensis) tissues. In this study, six candidate reference genes namely actin, Cyp2, GRAS, EgEfa1, F-box, and GLU were examined in leaf, node, internode, root, cabbage, shoot primordial and basal stem tissues of two-year old clonal oil palms. Two statistical algorithms, NormFinder and BestKeeper verified the stability of GRAS and Cyp2 reference genes in the oil palm tissues tested. The combination of Cyp2 and GRAS reference genes are recommended for gene expression normalisation profiles in nurserystage oil palm plantlets.
format Article
author A. H., Muhamad Afiq
Shaharuddin, Noor Azmi
R., Zubaidah
spellingShingle A. H., Muhamad Afiq
Shaharuddin, Noor Azmi
R., Zubaidah
Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
author_facet A. H., Muhamad Afiq
Shaharuddin, Noor Azmi
R., Zubaidah
author_sort A. H., Muhamad Afiq
title Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
title_short Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
title_full Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
title_fullStr Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
title_full_unstemmed Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
title_sort identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
publisher Malaysian Palm Oil Board
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80136/1/Identification%20of%20Reliable%20Reference%20Genes%20for%20Gene%20Expression%20Studies%20of%20Oil%20Palm%20Plantlets%20using%20NormFinder%20and%20BestKeeper%20Algorithms.pdf
http://psasir.upm.edu.my/id/eprint/80136/
_version_ 1680322363668824064
score 13.211869

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