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Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model

Vibriosis remains the main problem in aquaculture industry caused by bacteria from the genus Vibrio. This study was developed to identify and differentiate between the three Vibrio species which are Vibrio harveyi, V. alginolyticus and V. parahaemolyticus by PCR-outer membrane protein (ompW) gene. T...

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Main Authors: Z. Z., Nurfarizatul, N. K. P., Diyana, F. M., Amirah, Mohd Yaminudin, Nur Jasmin, Karim, M., Md Yasin, Ina Salwany
Format: Conference or Workshop Item
Language:English
Published: Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/77504/1/i-SIMBIOMAS%202016%2045.pdf
http://psasir.upm.edu.my/id/eprint/77504/
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spelling my.upm.eprints.775042020-04-15T16:43:17Z http://psasir.upm.edu.my/id/eprint/77504/ Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model Z. Z., Nurfarizatul N. K. P., Diyana F. M., Amirah Mohd Yaminudin, Nur Jasmin Karim, M. Md Yasin, Ina Salwany Vibriosis remains the main problem in aquaculture industry caused by bacteria from the genus Vibrio. This study was developed to identify and differentiate between the three Vibrio species which are Vibrio harveyi, V. alginolyticus and V. parahaemolyticus by PCR-outer membrane protein (ompW) gene. Three specific bands with size of 643 bp were produced and sequence analysis of three Vibrios used in this study showed high similarities of V. harveyi, V. alginolyticus and V. parahaemolyticus with published sequences, respectively. Thus, ompW gene can be used as phylomarker in differentiating Vibrio isolates. The effect of pathogenicity of local Vibrios strains with different concentrations on host-microbe interaction has been studied in the gnotobitic Artemia. After challenged, investigation revealed that maximum mortality of Artemia was observed at the concentration of 107 CFU/mL with V. parahaemolyticus have a highest mortality rate. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77504/1/i-SIMBIOMAS%202016%2045.pdf Z. Z., Nurfarizatul and N. K. P., Diyana and F. M., Amirah and Mohd Yaminudin, Nur Jasmin and Karim, M. and Md Yasin, Ina Salwany (2016) Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 45).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Vibriosis remains the main problem in aquaculture industry caused by bacteria from the genus Vibrio. This study was developed to identify and differentiate between the three Vibrio species which are Vibrio harveyi, V. alginolyticus and V. parahaemolyticus by PCR-outer membrane protein (ompW) gene. Three specific bands with size of 643 bp were produced and sequence analysis of three Vibrios used in this study showed high similarities of V. harveyi, V. alginolyticus and V. parahaemolyticus with published sequences, respectively. Thus, ompW gene can be used as phylomarker in differentiating Vibrio isolates. The effect of pathogenicity of local Vibrios strains with different concentrations on host-microbe interaction has been studied in the gnotobitic Artemia. After challenged, investigation revealed that maximum mortality of Artemia was observed at the concentration of 107 CFU/mL with V. parahaemolyticus have a highest mortality rate.
format Conference or Workshop Item
author Z. Z., Nurfarizatul
N. K. P., Diyana
F. M., Amirah
Mohd Yaminudin, Nur Jasmin
Karim, M.
Md Yasin, Ina Salwany
spellingShingle Z. Z., Nurfarizatul
N. K. P., Diyana
F. M., Amirah
Mohd Yaminudin, Nur Jasmin
Karim, M.
Md Yasin, Ina Salwany
Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model
author_facet Z. Z., Nurfarizatul
N. K. P., Diyana
F. M., Amirah
Mohd Yaminudin, Nur Jasmin
Karim, M.
Md Yasin, Ina Salwany
author_sort Z. Z., Nurfarizatul
title Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model
title_short Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model
title_full Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model
title_fullStr Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model
title_full_unstemmed Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model
title_sort molecular characterization of three vibrio species and study its pathogenic potential in a gnotobiotic artemia model
publisher Department of Biology, Faculty of Science, Universiti Putra Malaysia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/77504/1/i-SIMBIOMAS%202016%2045.pdf
http://psasir.upm.edu.my/id/eprint/77504/
_version_ 1665896030509465600
score 13.214268

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